C57BL/6JCya-Cptpem1/Cya
Common Name
Cptp-KO
Product ID
S-KO-15278
Backgroud
C57BL/6JCya
Strain ID
KOCMP-79554-Cptp-B6J-VA
When using this mouse strain in a publication, please cite “Cptp-KO Mouse (Catalog S-KO-15278) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cptp-KO
Strain ID
KOCMP-79554-Cptp-B6J-VA
Gene Name
Product ID
S-KO-15278
Gene Alias
Gltpd1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000030950
NCBI RefSeq
NM_024472
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Overview of Gene Research
Cptp, Ceramide-1-phosphate transfer protein, is a member of the glycolipid transfer protein (GLTP) superfamily. It shuttles ceramide-1-phosphate (C1P) between membranes, regulating cellular sphingolipid homeostasis. This function impacts programmed cell death, inflammation, autophagy, and inflammasome activation [1,3,4].
Knockdown of Cptp in human epithelial cells stimulates an 8-to 10-fold increase in autophagosomes, alters endogenous LC3-II and SQSTM1/p62 protein expression levels, and suppresses MTOR phosphorylation [3]. In THP-1 macrophage-like surveillance cells, Cptp knockdown not only induces autophagy but also elevates CASP1/caspase-1 levels and increases IL1B/interleukin-1β and IL18 release via a NLRP3-based mechanism [3]. In pancreatic cancer cells, CPTP is highly expressed, and its knockdown can inhibit cell growth and metastasis, suggesting it may function as a pro-tumorigenic gene [2].
In conclusion, Cptp is a crucial regulator of autophagy, inflammasome activation, and sphingolipid-related processes. Studies on Cptp, especially through loss-of-function models, have revealed its significance in inflammation-related diseases and cancer, providing potential therapeutic targets for these conditions.
References:
1. Gao, Yong-Guang, Zhai, Xiuhong, Boldyrev, Ivan A, Malinina, Lucy, Brown, Rhoderick E. 2021. Ceramide-1-phosphate transfer protein (CPTP) regulation by phosphoinositides. In The Journal of biological chemistry, 296, 100600. doi:10.1016/j.jbc.2021.100600. https://pubmed.ncbi.nlm.nih.gov/33781749/
2. Zhang, Yanqun, Ji, Shenying, Zhang, Xiangyu, Lam, Sin Man, Zou, Xianqiong. 2022. Human CPTP promotes growth and metastasis via sphingolipid metabolite ceramide and PI4KA/AKT signaling in pancreatic cancer cells. In International journal of biological sciences, 18, 4963-4983. doi:10.7150/ijbs.70007. https://pubmed.ncbi.nlm.nih.gov/35982909/
3. Mishra, Shrawan Kumar, Gao, Yong-Guang, Deng, Yibin, Hinchcliffe, Edward H, Brown, Rhoderick E. 2018. CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation. In Autophagy, 14, 862-879. doi:10.1080/15548627.2017.1393129. https://pubmed.ncbi.nlm.nih.gov/29164996/
4. Zhang, Yanqun, Zhang, Xiangyu, Lu, Mengyun, Zou, Xianqiong. 2021. Ceramide-1-phosphate and its transfer proteins in eukaryotes. In Chemistry and physics of lipids, 240, 105135. doi:10.1016/j.chemphyslip.2021.105135. https://pubmed.ncbi.nlm.nih.gov/34499882/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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