Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Med8em1/Cya
Common Name:
Med8-KO
Product ID:
S-KO-15295
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Med8-KO
Strain ID
KOCMP-80509-Med8-B6J-VA
Gene Name
Med8
Product ID
S-KO-15295
Gene Alias
2210021A15Rik; ARC32
Background
C57BL/6JCya
NCBI ID
80509
Modification
Conventional knockout
Chromosome
4
Phenotype
MGI:1915269
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Med8em1/Cya mice (Catalog S-KO-15295) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019229
NCBI RefSeq
NM_020000
Target Region
Exon 3~7
Size of Effective Region
~3.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
MED8, a subunit of the Mediator complex, is crucial for transcription as it links transcription factors to the RNA polymerase II transcriptional machinery [2,4,6]. It is involved in various biological processes across different organisms, highlighting its overall biological importance [1-5, 8-10]. Genetic models, such as gene-edited mutants in plants and knockdown models in cell lines, are valuable for studying MED8.

In Arabidopsis, loss-of-function of MED8 leads to a late-flowering phenotype due to increased expression of FLOWERING LOCUS C (FLC), as MED8 promotes floral transition by recruiting FPA to the FLC locus to repress its expression [1]. In tomato, overexpression of SlMED8 renders transgenic plants more tolerant to Botrytis cinerea, while antisense plants show compromised resistance, indicating its role in JA-dependent defense response [2]. In the diatom Phaeodactylum tricornutum, gene-editing mutants of MED8 exhibit decreased fucoxanthin content and reduced expression of key fucoxanthin-synthesis genes [3]. In hepatocellular carcinoma (HCC) cell lines, knockdown of MED8 weakens cell proliferation, colony-forming, and migration abilities [5]. High MED8 expression predicts poor disease-specific survival in HCC patients, and functional analysis shows enrichment in cell-cycle-related processes associated with MED8 expression [7].

In conclusion, MED8 is essential for multiple biological processes including floral transition, plant defense response, fucoxanthin synthesis, and is associated with HCC prognosis. Studies using gene-edited mutants and knockdown models have revealed its role in these processes, contributing to our understanding of plant development, stress response, and cancer-related mechanisms.

References:

1. Yuan, Chen, Hu, Yikai, Liu, Qinggang, Shen, Lisha, Qin, Cheng. 2023. MED8 regulates floral transition in Arabidopsis by interacting with FPA. In The Plant journal : for cell and molecular biology, 116, 1234-1247. doi:10.1111/tpj.16419. https://pubmed.ncbi.nlm.nih.gov/37565662/

2. Zhang, Lili, Song, Yunpeng, Liu, Kaige, Gong, Fanrong. 2021. The tomato Mediator subunit MED8 positively regulates plant response to Botrytis cinerea. In Journal of plant physiology, 266, 153533. doi:10.1016/j.jplph.2021.153533. https://pubmed.ncbi.nlm.nih.gov/34601339/

3. Zhao, Hejing, Liu, Yan, Zhu, Zhengjiang, Yan, Xiaojun, Li, Xiaohui. 2023. Mediator subunit MED8 interacts with heat shock transcription factor HSF3 to promote fucoxanthin synthesis in the diatom Phaeodactylum tricornutum. In The New phytologist, 241, 1574-1591. doi:10.1111/nph.19467. https://pubmed.ncbi.nlm.nih.gov/38062856/

4. Mehta, Surbhi, Miklos, Ida, Sipiczki, Matthias, Sengupta, Sagar, Sharma, Nimisha. 2009. The Med8 mediator subunit interacts with the Rpb4 subunit of RNA polymerase II and Ace2 transcriptional activator in Schizosaccharomyces pombe. In FEBS letters, 583, 3115-20. doi:10.1016/j.febslet.2009.08.036. https://pubmed.ncbi.nlm.nih.gov/19720063/

5. Jin, Xiaojun, Song, Yongfei, An, Zhanglu, Lu, Hongsheng, Lian, Jiangfang. 2022. A Predictive Model for Prognosis and Therapeutic Response in Hepatocellular Carcinoma Based on a Panel of Three MED8-Related Immunomodulators. In Frontiers in oncology, 12, 868411. doi:10.3389/fonc.2022.868411. https://pubmed.ncbi.nlm.nih.gov/35558516/

6. Chaves, R S, Herrero, P, Moreno, F. . Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes. In Biochemical and biophysical research communications, 254, 345-50. doi:. https://pubmed.ncbi.nlm.nih.gov/9918841/

7. Wu, Shuang, Li, Qiao, Cao, Yuan, Wang, Zengguo, Zhang, Taoyuan. 2022. Mediator complex subunit 8 is a prognostic biomarker in hepatocellular carcinoma. In American journal of translational research, 14, 1765-1777. doi:. https://pubmed.ncbi.nlm.nih.gov/35422940/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest