C57BL/6JCya-Gpr84em1/Cya
Common Name:
Gpr84-KO
Product ID:
S-KO-15339
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpr84-KO
Strain ID
KOCMP-80910-Gpr84-B6J-VA
Gene Name
Product ID
S-KO-15339
Gene Alias
EX33
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr84em1/Cya mice (Catalog S-KO-15339) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000079824
NCBI RefSeq
NM_030720
Target Region
Exon 2
Size of Effective Region
~0.3 kb
Detailed Document
Overview of Gene Research
Gpr84, a G protein-coupled receptor, is a medium-chain fatty acid receptor. It is involved in multiple physiological and pathological processes. Gpr84 signaling is related to inflammatory pathways and nutrient-sensing in metabolism, potentially modulating food intake [6].
In loss-of-function studies, Gpr84-KO mice showed increased lipid accumulation and reduced activity in brown adipose tissue (BAT), making them more vulnerable to cold. The in vitro experiments with primary brown adipocytes from Gpr84-KO mice indicated diminished thermogenic gene expression and reduced O₂ consumption. These findings suggest mitochondrial dysfunction as the cause of BAT anomalies in Gpr84-KO mice, highlighting Gpr84 as a potential target for metabolic disorder treatment [1].
In acute lung injury (ALI), Gpr84 deficiency or blockage significantly ameliorated lung inflammation in mice by reducing neutrophil infiltration and oxidative stress. Gpr84 -/- mice were also resistant to dextran sulfate sodium-induced colitis, with Gpr84 activation promoting pro-inflammatory properties in colonic macrophages [2,3].
In cancer, Gpr84 is overexpressed on myeloid-derived suppressor cells (MDSCs), and Gpr84 antagonism combined with an anti-PD-1 antibody enhanced antitumor responses. Additionally, Gpr84 transferred from MDSCs to CD8⁺ T cells via exosomes induced T-cell senescence through the p53 signaling pathway, suppressing the antitumor response [4,5].
In summary, Gpr84 plays crucial roles in metabolism, inflammation, and cancer-related immune responses. Gene knockout mouse models have revealed its significance in these biological processes and disease conditions, suggesting Gpr84 as a potential therapeutic target for metabolic disorders, ALI, ulcerative colitis, and enhancing anti-PD-1 immunotherapy efficacy in cancer [1-5].
References:
1. Sun, Xue-Nan, An, Yu A, Paschoal, Vivian A, Gupta, Rana K, Oh, Da Young. 2023. GPR84-mediated signal transduction affects metabolic function by promoting brown adipocyte activity. In The Journal of clinical investigation, 133, . doi:10.1172/JCI168992. https://pubmed.ncbi.nlm.nih.gov/37856216/
2. Wang, Si-Wei, Zhang, Qing, Lu, Dan, Nan, Fa-Jun, Xie, Xin. 2023. GPR84 regulates pulmonary inflammation by modulating neutrophil functions. In Acta pharmacologica Sinica, 44, 1665-1675. doi:10.1038/s41401-023-01080-z. https://pubmed.ncbi.nlm.nih.gov/37016043/
3. Zhang, Qing, Chen, Lin-Hai, Yang, Hui, Nan, Fa-Jun, Xie, Xin. 2021. GPR84 signaling promotes intestinal mucosal inflammation via enhancing NLRP3 inflammasome activation in macrophages. In Acta pharmacologica Sinica, 43, 2042-2054. doi:10.1038/s41401-021-00825-y. https://pubmed.ncbi.nlm.nih.gov/34912006/
4. Qin, Guohui, Liu, Shasha, Liu, Jinyan, Zhang, Bin, Zhang, Yi. 2023. Overcoming resistance to immunotherapy by targeting GPR84 in myeloid-derived suppressor cells. In Signal transduction and targeted therapy, 8, 164. doi:10.1038/s41392-023-01388-6. https://pubmed.ncbi.nlm.nih.gov/37105980/
5. Liu, Jinyan, Liu, Jiayin, Qin, Guohui, Wang, Liping, Zhang, Yi. 2023. MDSCs-derived GPR84 induces CD8+ T-cell senescence via p53 activation to suppress the antitumor response. In Journal for immunotherapy of cancer, 11, . doi:10.1136/jitc-2023-007802. https://pubmed.ncbi.nlm.nih.gov/38016719/
6. Aktar, Rubina, Rondinelli, Silvia, Peiris, Madusha. 2023. GPR84 in physiology-Many functions in many tissues. In British journal of pharmacology, 181, 1524-1535. doi:10.1111/bph.16206. https://pubmed.ncbi.nlm.nih.gov/37533166/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen