C57BL/6JCya-Rnf114em1/Cya
Common Name:
Rnf114-KO
Product ID:
S-KO-15353
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rnf114-KO
Strain ID
KOCMP-81018-Rnf114-B6J-VA
Gene Name
Product ID
S-KO-15353
Gene Alias
1110008J21Rik; Zfp228; Zfp313; Znf228
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rnf114em1/Cya mice (Catalog S-KO-15353) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109214
NCBI RefSeq
NM_030743.6
Target Region
Exon 3~8
Size of Effective Region
~12.2 kb
Detailed Document
Overview of Gene Research
RNF114, also known as E3 ubiquitin ligase RING finger protein 114, is an E3 ubiquitin ligase involved in multiple biological processes. It participates in the DNA damage response as a PARylation-dependent enzyme, where it targets PARP1 for degradation [1]. It is also associated with the regulation of cell proliferation, metastasis in cancers, and early embryo development [2,3,4,5]. Genetic models such as gene knockout could potentially help further elucidate its functions.
In loss-of-function studies, silencing RNF114 in gastric cancer cells suppressed their proliferation and metastasis, suggesting its oncogenic role in this cancer type [2]. In colorectal cancer, knockdown of RNF114 diminished the proliferation, stemness, invasion, and wound healing of cancer cells, and also facilitated apoptosis both in vitro and in vivo [3]. Depletion of maternal RNF114 in mouse embryos led to developmental arrest at the two-cell stage, indicating its crucial role in zygotic genome activation [4].
In conclusion, RNF114 is an important E3 ubiquitin ligase involved in DNA damage response, cancer cell behaviors, and embryo development. Studies using gene knockout or knockdown models have revealed its oncogenic role in gastric and colorectal cancers, as well as its significance in mouse embryo development, providing potential therapeutic targets for cancer treatment and insights into embryo development mechanisms.
References:
1. Li, Peng, Zhen, Yuanli, Kim, Chiho, Qin, Tian, Yu, Yonghao. 2023. Nimbolide targets RNF114 to induce the trapping of PARP1 and synthetic lethality in BRCA-mutated cancer. In Science advances, 9, eadg7752. doi:10.1126/sciadv.adg7752. https://pubmed.ncbi.nlm.nih.gov/37878693/
2. Feng, Zongfeng, Li, Leyan, Zeng, Qingwen, Cao, Yi, Li, Zhengrong. 2022. RNF114 Silencing Inhibits the Proliferation and Metastasis of Gastric Cancer. In Journal of Cancer, 13, 565-578. doi:10.7150/jca.62033. https://pubmed.ncbi.nlm.nih.gov/35069903/
3. Liu, Shi-Cheng, Gong, Le-Lan, Huang, Feng-Chang, Liu, Xi-Hong, Li, Wen-Liang. 2023. RNF114 facilitates the proliferation, stemness, and metastasis of colorectal cancer. In Pathology, research and practice, 248, 154716. doi:10.1016/j.prp.2023.154716. https://pubmed.ncbi.nlm.nih.gov/37523804/
4. Zhou, Shuai, Guo, Yueshuai, Sun, Haifeng, Guo, Xuejiang, Huo, Ran. 2021. Maternal RNF114-mediated target substrate degradation regulates zygotic genome activation in mouse embryos. In Development (Cambridge, England), 148, . doi:10.1242/dev.199426. https://pubmed.ncbi.nlm.nih.gov/34104941/
5. da Silva, Zigomar, Glanzner, Werner Giehl, Gutierrez, Karina, Gonçalves, Paulo Bayard Dias, Bordignon, Vilceu. 2023. DCAF13 and RNF114 participate in the regulation of early porcine embryo development. In Reproduction (Cambridge, England), 166, 401-410. doi:10.1530/REP-23-0230. https://pubmed.ncbi.nlm.nih.gov/37855431/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen