C57BL/6NCya-Mcamem1/Cya
Common Name
Mcam-KO
Product ID
S-KO-15421
Backgroud
C57BL/6NCya
Strain ID
KOCMP-84004-Mcam-B6N-VA
Status
When using this mouse strain in a publication, please cite “Mcam-KO Mouse (Catalog S-KO-15421) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mcam-KO
Strain ID
KOCMP-84004-Mcam-B6N-VA
Gene Name
Product ID
S-KO-15421
Gene Alias
CD146, CD149, Muc18, s-endo, 1-gicerin, s-gicerin
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000034650
NCBI RefSeq
NM_023061
Target Region
Exon 2~15
Size of Effective Region
~4.9 kb
Overview of Gene Research
Mcam, also known as Melanoma cell adhesion molecule (CD146/MUC18), is an adhesion molecule of the immunoglobulin superfamily. It plays roles in cell-cell adhesion and is involved in multiple biological processes, especially angiogenesis, and is associated with the Wnt signaling pathway [3]. Genetic models, such as knockout mice, are valuable for studying its functions.
In mammary gland development, Mcam knockout in mice increases the clonogenicity and regenerative capacity of mammary gland epithelial cells (MECs). It promotes MEC proliferation, differentiation, and ductal morphogenesis by recruiting and polarizing macrophages through the Il4-Stat6 axis, which in turn promotes secretion of the non-canonical Wnt ligand Wnt5a and its binding to the receptor Ryk [1]. In colorectal carcinogenesis, stromal Mcam knockout in mice attenuates orthotopically injected colorectal tumoroid growth and improves survival by decreasing tumor-associated macrophage recruitment. Fibroblast MCAM interacts with interleukin-1 receptor 1 to augment nuclear factor κB-IL34/CCL8 signaling that promotes macrophage chemotaxis [2].
In conclusion, Mcam is crucial in regulating cell-related functions in processes like mammary gland development and colorectal carcinogenesis. Gene knockout mouse models have revealed its role in promoting or inhibiting cell growth and immune cell recruitment in these contexts, providing insights into potential therapeutic targets for related diseases.
References:
1. Yang, Xing, Xu, Haibo, Yang, Xu, Hou, Zongliu, Jiao, Baowei. 2024. Mcam inhibits macrophage-mediated development of mammary gland through non-canonical Wnt signaling. In Nature communications, 15, 36. doi:10.1038/s41467-023-44338-0. https://pubmed.ncbi.nlm.nih.gov/38167296/
2. Kobayashi, Hiroki, Gieniec, Krystyna A, Lannagan, Tamsin R M, Woods, Susan L, Worthley, Daniel L. 2021. The Origin and Contribution of Cancer-Associated Fibroblasts in Colorectal Carcinogenesis. In Gastroenterology, 162, 890-906. doi:10.1053/j.gastro.2021.11.037. https://pubmed.ncbi.nlm.nih.gov/34883119/
3. Joshkon, Ahmad, Heim, Xavier, Dubrou, Cléa, Bardin, Nathalie, Blot-Chabaud, Marcel. 2020. Role of CD146 (MCAM) in Physiological and Pathological Angiogenesis-Contribution of New Antibodies for Therapy. In Biomedicines, 8, . doi:10.3390/biomedicines8120633. https://pubmed.ncbi.nlm.nih.gov/33352759/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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