C57BL/6NCya-Bcl2l13em1/Cya
Common Name:
Bcl2l13-KO
Product ID:
S-KO-15521
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bcl2l13-KO
Strain ID
KOCMP-94044-Bcl2l13-B6N-VA
Gene Name
Product ID
S-KO-15521
Gene Alias
BCL-RAMBO; E430016C20Rik; Mil-1; Mil1
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Bcl2l13em1/Cya mice (Catalog S-KO-15521) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009256
NCBI RefSeq
NM_153516
Target Region
Exon 3~4
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
BCL2L13, a BCL2-like protein, has been studied for about two decades and is emerging as a research hotspot. It anchors in the mitochondrial outer membrane, expresses in various cells and tissues, and is involved in multiple processes. It cannot be simply classified as a pro-or anti-apoptotic protein as it can induce mitochondrial fragmentation, inhibit cell death, promote mitophagy, and is associated with growth, development, energy metabolism [1].
In glioblastoma, BCL2L13 expression is upregulated. It targets DNM1L at the Ser616 site, leading to mitochondrial fission and high mitophagy flux, which promotes the proliferation and invasion of glioblastoma cells both in vitro and in vivo, suggesting it as a candidate molecular target for treating glioblastoma [2]. In zebrafish, loss of Bcl2l13 impairs skeletal muscle structure and function, alters Ca2+ signaling, and decreases mitochondrial complexes activity. In mammalian cells, BCL2L13 is present at mitochondria, ER, and mitochondria-associated membranes, and its knockdown in C2C12 cells changes cytosolic Ca2+ release and mitochondrial Ca2+ uptake, indicating its role in maintaining proper skeletal muscle function through Ca2+ signaling [3].
In conclusion, BCL2L13 is crucial for multiple biological processes. Its functions in promoting mitophagy in glioblastoma and maintaining skeletal muscle function through Ca2+ signaling regulation are significant. Research on BCL2L13, especially through gene-knockout or conditional-knockout models as seen in zebrafish, helps in understanding its role in specific disease conditions like glioblastoma, providing potential targets for treatment.
References:
1. Meng, Fei, Sun, Naitong, Liu, Dongyan, Xiao, Jun, Dai, Haiming. 2020. BCL2L13: physiological and pathological meanings. In Cellular and molecular life sciences : CMLS, 78, 2419-2428. doi:10.1007/s00018-020-03702-9. https://pubmed.ncbi.nlm.nih.gov/33201252/
2. Wang, Jiwei, Chen, Anbin, Xue, Zhiwei, Wang, Xinyu, Huang, Bin. 2023. BCL2L13 promotes mitophagy through DNM1L-mediated mitochondrial fission in glioblastoma. In Cell death & disease, 14, 585. doi:10.1038/s41419-023-06112-4. https://pubmed.ncbi.nlm.nih.gov/37660127/
3. Grepper, Dogan, Tabasso, Cassandra, Zanou, Nadège, Fajas, Lluis, Amati, Francesca. 2024. BCL2L13 at endoplasmic reticulum-mitochondria contact sites regulates calcium homeostasis to maintain skeletal muscle function. In iScience, 27, 110510. doi:10.1016/j.isci.2024.110510. https://pubmed.ncbi.nlm.nih.gov/39175772/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen