C57BL/6NCya-Ptges2em1/Cya
Common Name:
Ptges2-KO
Product ID:
S-KO-15580
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ptges2-KO
Strain ID
KOCMP-96979-Ptges2-B6N-VA
Gene Name
Product ID
S-KO-15580
Gene Alias
0610038H10Rik; Gbf1; Mpges2; Pges2
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ptges2em1/Cya mice (Catalog S-KO-15580) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028162
NCBI RefSeq
NM_133783
Target Region
Exon 1~7
Size of Effective Region
~6.1 kb
Detailed Document
Overview of Gene Research
Ptges2, also known as microsomal prostaglandin E synthase-2 (mPGES-2), is a key enzyme in the synthesis of prostaglandin E2 (PGE2) from COX-derived PGH2 [3]. It is involved in multiple biological pathways, such as the arachidonic acid metabolism pathway [4]. PGE2, the product of Ptges2-catalyzed reaction, has a wide range of biological activities and is involved in inflammation, fever, and other physiological processes. Genetic models, like gene knockout mice, have been crucial in studying Ptges2's function.
In mouse models, global or tubule-specific knockout of Ptges2 led to decreased renal dysfunction and morphological damage induced by cisplatin and unilateral renal ischemia/reperfusion, suggesting that blocking Ptges2 may be a promising therapeutic strategy for acute kidney injury (AKI) as it inhibits ferroptosis via the heme-dependent regulation of the p53/SLC7A11/GPX4 axis [1]. Also, global knockout or pharmacological blockage of Ptges2 in mice attenuated diabetic podocyte injury and tubulointerstitial fibrosis, alleviating lipid accumulation and lipotoxicity in diabetic kidney disease (DKD), as mPGES-2 and Rev-Erbα compete for heme binding to regulate fatty acid binding protein 5 expression and lipid metabolism in the diabetic kidney [2].
In conclusion, Ptges2 is essential for PGE2 synthesis and is involved in important biological processes. The study of Ptges2 using KO mouse models has revealed its significance in diseases like AKI and DKD, providing potential therapeutic directions for these conditions.
References:
1. Zhong, Dandan, Quan, Lingling, Hao, Chang, Jia, Zhanjun, Sun, Ying. 2023. Targeting mPGES-2 to protect against acute kidney injury via inhibition of ferroptosis dependent on p53. In Cell death & disease, 14, 710. doi:10.1038/s41419-023-06236-7. https://pubmed.ncbi.nlm.nih.gov/37907523/
2. Zhong, Dandan, Chen, Jingshuo, Qiao, Ranran, Jia, Zhanjun, Sun, Ying. 2024. Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling. In Cell reports, 43, 114075. doi:10.1016/j.celrep.2024.114075. https://pubmed.ncbi.nlm.nih.gov/38583151/
3. Nakatani, Yoshihito, Kudo, Ichiro. . [Prostaglandin E2 synthases]. In Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 120, 373-8. doi:. https://pubmed.ncbi.nlm.nih.gov/12528468/
4. Zhao, Zhibo, Liu, Xinyi, Xiang, Yue, Gong, Jianping, Zhao, Lei. 2023. Inhibiting cholesterol de novo synthesis promotes hepatocellular carcinoma progression by upregulating prostaglandin E synthase 2-mediated arachidonic acid metabolism under high fatty acid conditions. In Cancer science, 115, 477-489. doi:10.1111/cas.16035. https://pubmed.ncbi.nlm.nih.gov/38081591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen