C57BL/6JCya-Lgi1em1/Cya
Common Name
Lgi1-KO
Product ID
S-KO-15701
Backgroud
C57BL/6JCya
Strain ID
KOCMP-56839-Lgi1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Lgi1-KO Mouse (Catalog S-KO-15701) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Lgi1-KO
Strain ID
KOCMP-56839-Lgi1-B6J-VA
Gene Name
Product ID
S-KO-15701
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000198518
NCBI RefSeq
NM_020278
Target Region
Exon 3~7
Size of Effective Region
~17.4 kb
Overview of Gene Research
Lgi1, or Leucine-rich glioma-inactivated protein 1, is a secreted glycoprotein mainly expressed in the brain, playing a crucial role in central nervous system development and physiology. It is associated with pathways related to myelination, synaptic transmission, and regulation of neuronal activity. Mutations or auto-antibodies against LGI1 are linked to autosomal dominant lateral temporal lobe epilepsy (ADLTE) and autoimmune encephalitis respectively, highlighting its importance in maintaining normal brain function [2].
Conditional knockout (cKO) of Opalin, an identified LGI1 receptor on oligodendrocytes (OLs), caused hypomyelination and white matter abnormalities, phenocopying LGI1 deficiency in mice. This indicates that the LGI1/OPALIN complex is vital for OL differentiation and myelination [1]. Knock-down of LGI1 in rodents revealed its necessity for normal brain development. In mature brains, it associates with Kv1 channels and AMPA receptors, regulating their expression and function, and its loss-of-function is a common feature in the genesis of epileptic seizures in ADLTE and anti-LGI1 autoimmune encephalitis [2].
In summary, Lgi1 is essential for normal brain development, myelination, and regulation of synaptic transmission. Studies using KO/CKO mouse models have significantly contributed to understanding its role in diseases such as epilepsy and autoimmune encephalitis, providing insights into the underlying molecular mechanisms and potential therapeutic targets.
References:
1. Teng, Xiao-Yu, Hu, Ping, Zhang, Cai-Ming, Chen, Guiquan, Shi, Yun Stone. 2024. OPALIN is an LGI1 receptor promoting oligodendrocyte differentiation. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2403652121. doi:10.1073/pnas.2403652121. https://pubmed.ncbi.nlm.nih.gov/39083419/
2. Baudin, Paul, Cousyn, Louis, Navarro, Vincent. 2021. The LGI1 protein: molecular structure, physiological functions and disruption-related seizures. In Cellular and molecular life sciences : CMLS, 79, 16. doi:10.1007/s00018-021-04088-y. https://pubmed.ncbi.nlm.nih.gov/34967933/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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