C57BL/6JCya-Ccn5em1/Cya
Common Name:
Ccn5-KO
Product ID:
S-KO-16036
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ccn5-KO
Strain ID
KOCMP-22403-Ccn5-B6J-VB
Gene Name
Product ID
S-KO-16036
Gene Alias
Crgr4; Ctgfl; Rcop1; Wisp2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccn5em1/Cya mice (Catalog S-KO-16036) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029188
NCBI RefSeq
NM_016873.2
Target Region
Exon 3
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Ccn5, also known as WNT1 inducible signaling pathway protein 2 (WISP2), is a matricellular protein. Its expression is regulated by Wnt signaling and IGF-1. Ccn5 is involved in multiple biological processes. It acts on mesenchymal stem cell (MSCs) proliferation and differentiation, and is an important regulator of fibrosis. It participates in both the canonical WNT and the TGFβ pathways [1,3].
Systemic deficiency of Ccn5 gene expression in mice led to adipocyte hypertrophy, increased adipogenesis, lipid accumulation, insulin resistance, and glucose intolerance, which were exacerbated by high-fat diet feeding. On the other hand, adipocyte-specific and systemic overexpression of Ccn5 caused an increase in lean body mass, improved insulin sensitivity, hyperplasia of cardiomyocytes, increased heart mass, and decreased fasting glucose levels, indicating its role as a regulator of adipocyte proliferation and maturation, affecting lean/fat mass ratio and insulin sensitivity [2].
In conclusion, Ccn5 is a crucial regulator in metabolic processes. The study of Ccn5 gene-deficient and overexpressing mouse models has revealed its significant role in metabolic diseases such as obesity and diabetes, providing insights into potential treatment strategies for these conditions.
References:
1. Grünberg, John R, Elvin, Johannes, Paul, Alexandra, Hammarstedt, Ann, Smith, Ulf. 2017. CCN5/WISP2 and metabolic diseases. In Journal of cell communication and signaling, 12, 309-318. doi:10.1007/s12079-017-0437-z. https://pubmed.ncbi.nlm.nih.gov/29247377/
2. Alami, Tara, Liu, Jun-Li. 2021. Metabolic Effects of CCN5/WISP2 Gene Deficiency and Transgenic Overexpression in Mice. In International journal of molecular sciences, 22, . doi:10.3390/ijms222413418. https://pubmed.ncbi.nlm.nih.gov/34948212/
3. Liu, Jun-Li, Kaddour, Nancy, Chowdhury, Subrata, Li, Qing, Gao, Zu-Hua. 2016. Role of CCN5 (WNT1 inducible signaling pathway protein 2) in pancreatic islets. In Journal of diabetes, 9, 462-474. doi:10.1111/1753-0407.12507. https://pubmed.ncbi.nlm.nih.gov/27863006/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen