C57BL/6JCya-Slc25a5em1/Cya
Common Name:
Slc25a5-KO
Product ID:
S-KO-16226
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Slc25a5-KO
Strain ID
KOCMP-11740-Slc25a5-B6J-VB
Gene Name
Product ID
S-KO-16226
Gene Alias
Ant2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc25a5em1/Cya mice (Catalog S-KO-16226) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016463
NCBI RefSeq
NM_007451
Target Region
Exon 2
Size of Effective Region
~0.5 kb
Detailed Document
Overview of Gene Research
Slc25a5, also known as solute carrier family 25 (mitochondrial carrier, adenine nucleotide translocator), member 5 or ANT2, is a critical inner mitochondrial membrane protein. It functions as a carrier for metabolites, playing a key role in mitochondrial energy metabolism, such as ATP production [2,5]. It is involved in pathways like PINK1-PRKN-dependent mitophagy [2]. Genetic models can be used to study its function and impact on biological processes and diseases.
In colon cancer, Slc25a5 was downregulated, and its upregulation was related to better overall survival. It attenuated cell proliferation, upregulated programmed cell death-related signatures, and inhibited the MAPK signaling pathway [1]. In diabetes-induced osteopenia, BK channels regulated Slc25a5-mediated mitophagy in osteoblasts [2]. In adipogenesis in OP9 cells, depletion of Slc25a5 suppressed adipogenesis-related genes, triglyceride accumulation, and PPARγ protein expression, and reduced oxidative phosphorylation and ATP production, potentially mediated by ERK1/2 phosphorylation [3]. In neuroblastoma, high expression of Slc25a5 predicted poor patient prognosis, and inhibiting it with PENAO reduced cell viability. The combination of PENAO and the histone deacetylase inhibitor SAHA synergistically reduced cell viability and induced apoptosis in neuroblastoma cells [4].
In summary, Slc25a5 is crucial for mitochondrial-related functions like energy metabolism and mitophagy. Its study through model-based research has revealed its role in diseases such as colon cancer, diabetes-induced osteopenia, adipogenesis-related obesity, and neuroblastoma, providing insights into disease mechanisms and potential therapeutic targets.
References:
1. Chen, Yan-Jie, Hong, Wei-Feng, Liu, Meng-Ling, Liu, Tian-Shu, Liang, Li. 2022. An integrated bioinformatic investigation of mitochondrial solute carrier family 25 (SLC25) in colon cancer followed by preliminary validation of member 5 (SLC25A5) in tumorigenesis. In Cell death & disease, 13, 237. doi:10.1038/s41419-022-04692-1. https://pubmed.ncbi.nlm.nih.gov/35288533/
2. Jiang, Lan, He, Haidong, Tang, Yuyan, Cai, Hui, Zhang, Xuemei. 2024. Activation of BK channels prevents diabetes-induced osteopenia by regulating mitochondrial Ca2+ and SLC25A5/ANT2-PINK1-PRKN-mediated mitophagy. In Autophagy, 20, 2388-2404. doi:10.1080/15548627.2024.2367184. https://pubmed.ncbi.nlm.nih.gov/38873928/
3. Zhu, Shenglong, Wang, Wei, Zhang, Jingwei, Jing, Zhe, Chen, Yong Q. 2022. Slc25a5 regulates adipogenesis by modulating ERK signaling in OP9 cells. In Cellular & molecular biology letters, 27, 11. doi:10.1186/s11658-022-00314-y. https://pubmed.ncbi.nlm.nih.gov/35109789/
4. Seneviratne, Janith A, Carter, Daniel R, Mittra, Rituparna, Cheung, Belamy B, Marshall, Glenn M. 2022. Inhibition of mitochondrial translocase SLC25A5 and histone deacetylation is an effective combination therapy in neuroblastoma. In International journal of cancer, 152, 1399-1413. doi:10.1002/ijc.34349. https://pubmed.ncbi.nlm.nih.gov/36346110/
5. Le, Jiamei, Chen, Yilong, Yang, Wei, Chen, Ligong, Ye, Jianping. 2023. Metabolic basis of solute carrier transporters in treatment of type 2 diabetes mellitus. In Acta pharmaceutica Sinica. B, 14, 437-454. doi:10.1016/j.apsb.2023.09.004. https://pubmed.ncbi.nlm.nih.gov/38322335/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen