C57BL/6JCya-Smad3em1/Cya
Common Name
Smad3-KO
Product ID
S-KO-16227
Backgroud
C57BL/6JCya
Strain ID
KOCMP-17127-Smad3-B6J-VA
When using this mouse strain in a publication, please cite “Smad3-KO Mouse (Catalog S-KO-16227) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Smad3-KO
Strain ID
KOCMP-17127-Smad3-B6J-VA
Gene Name
Product ID
S-KO-16227
Gene Alias
Madh3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000034973
NCBI RefSeq
NM_016769
Target Region
Exon 2~3
Size of Effective Region
~0.4 kb
Overview of Gene Research
Smad3 is a key mediator of the TGF-β signaling pathway. It plays a crucial role in multiple biological processes such as cell proliferation, differentiation, tissue repair, and fibrosis [1,2,3,4]. It can interact with other signaling pathways like ERK/p38 MAPK and NF-κB, and is also involved in regulating many downstream target genes including non-coding RNAs [1].
Smad3-null mice have been used to study its functions. These mice are resistant to various types of fibrosis, including radiation-induced cutaneous fibrosis, bleomycin-induced pulmonary fibrosis, carbon tetrachloride-induced hepatic fibrosis, and glomerular fibrosis induced by type 1 diabetes [3]. In fibrotic conditions resulting from epithelial-to-mesenchymal transdifferentiation (EMT), such as proliferative vitreoretinopathy, ocular capsule injury, and glomerulosclerosis from unilateral ureteral obstruction, Smad3-null mice show an abrogated fibrotic response [3].
In conclusion, Smad3 is essential in the TGF-β signaling pathway and significantly contributes to fibrosis in multiple organs. The use of Smad3-null mouse models has provided valuable insights into its role in fibrotic diseases, highlighting its potential as a therapeutic target for treating pathological fibrotic conditions [3].
References:
1. Wu, Wenjing, Wang, Xiaoqin, Yu, Xueqing, Lan, Hui-Yao. 2022. Smad3 Signatures in Renal Inflammation and Fibrosis. In International journal of biological sciences, 18, 2795-2806. doi:10.7150/ijbs.71595. https://pubmed.ncbi.nlm.nih.gov/35541902/
2. Yamaguchi, Takashi, Yoshida, Katsunori, Murata, Miki, Matsuzaki, Koichi, Naganuma, Makoto. 2022. Smad3 Phospho-Isoform Signaling in Nonalcoholic Steatohepatitis. In International journal of molecular sciences, 23, . doi:10.3390/ijms23116270. https://pubmed.ncbi.nlm.nih.gov/35682957/
3. Flanders, Kathleen C. . Smad3 as a mediator of the fibrotic response. In International journal of experimental pathology, 85, 47-64. doi:. https://pubmed.ncbi.nlm.nih.gov/15154911/
4. Gao, Zhen. 2025. New insights into Smad3 in cardiac fibrosis. In Gene, 952, 149418. doi:10.1016/j.gene.2025.149418. https://pubmed.ncbi.nlm.nih.gov/40089084/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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