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C57BL/6JCya-Chtopem1/Cya
Common Name:
Chtop-KO
Product ID:
S-KO-16232
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chtop-KO
Strain ID
KOCMP-66511-Chtop-B6J-VA
Gene Name
Chtop
Product ID
S-KO-16232
Gene Alias
2500003M10Rik; Fop; Srag
Background
C57BL/6JCya
NCBI ID
66511
Modification
Conventional knockout
Chromosome
3
Phenotype
MGI:1913761
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chtopem1/Cya mice (Catalog S-KO-16232) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001043
NCBI RefSeq
NM_023215
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Chtop, short for Chromatin target of protein arginine methyltransferase, is a versatile regulator involved in gene-specific transcription and mRNA export. It binds competitively to arginine methyltransferases PRMT1 and PRMT5, promoting asymmetric or symmetric methylation of arginine residues respectively. Chtop is part of the TREX complex, linking transcription elongation to mRNA export, and is crucial for the expression of certain gene groups and in the pathogenesis of some diseases [1,4].

In chemoresistant epithelial ovarian cancer (EOC), Chtop is highly expressed, and its knockdown enhances cisplatin-induced apoptosis, reduces cell stemness, and decreases metastatic potential, suggesting it as a potential therapeutic target [2]. In microglia, LPS stimulation induces Chtop expression. Knocking down Chtop decreases pro-inflammatory cytokine expression, alters cell metabolism, and alleviates microglia-mediated neuroinflammation [3]. Chtop also auto-regulates its expression level via intron retention and nonsense-mediated decay of its own mRNA [5].

In conclusion, Chtop is essential in regulating gene-specific expression, mRNA export, and cell-specific functions. Its study in models provides insights into diseases like chemoresistant EOC and microglia-mediated neuroinflammation, highlighting its potential as a therapeutic target in these disease areas.

References:

1. Izumikawa, Keiichi, Ishikawa, Hideaki, Simpson, Richard J, Takahashi, Nobuhiro. 2018. Modulating the expression of Chtop, a versatile regulator of gene-specific transcription and mRNA export. In RNA biology, 15, 849-855. doi:10.1080/15476286.2018.1465795. https://pubmed.ncbi.nlm.nih.gov/29683372/

2. Feng, Xiaojie, Bai, Xupeng, Ni, Jie, Graham, Peter, Li, Yong. 2019. CHTOP in Chemoresistant Epithelial Ovarian Cancer: A Novel and Potential Therapeutic Target. In Frontiers in oncology, 9, 557. doi:10.3389/fonc.2019.00557. https://pubmed.ncbi.nlm.nih.gov/31380263/

3. Zhou, Xin, Lv, Mengfei, Duan, Zhongying, Liu, Jiake, Cui, Yu. . CHTOP Promotes Microglia-Mediated Inflammation by Regulating Cell Metabolism and Inflammatory Gene Expression. In Journal of immunology (Baltimore, Md. : 1950), 212, 677-688. doi:10.4049/jimmunol.2300572. https://pubmed.ncbi.nlm.nih.gov/38117276/

4. Chang, Chung-Te, Hautbergue, Guillaume M, Walsh, Matthew J, Philipsen, Sjaak, Wilson, Stuart A. 2013. Chtop is a component of the dynamic TREX mRNA export complex. In The EMBO journal, 32, 473-86. doi:10.1038/emboj.2012.342. https://pubmed.ncbi.nlm.nih.gov/23299939/

5. Izumikawa, Keiichi, Yoshikawa, Harunori, Ishikawa, Hideaki, Isobe, Toshiaki, Takahashi, Nobuhiro. 2016. Chtop (Chromatin target of Prmt1) auto-regulates its expression level via intron retention and nonsense-mediated decay of its own mRNA. In Nucleic acids research, 44, 9847-9859. doi:. https://pubmed.ncbi.nlm.nih.gov/27683223/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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