C57BL/6JCya-Cacna1fem1/Cya
Common Name
Cacna1f-KO
Product ID
S-KO-16388
Backgroud
C57BL/6JCya
Strain ID
KOCMP-54652-Cacna1f-B6J-VB
When using this mouse strain in a publication, please cite “Cacna1f-KO Mouse (Catalog S-KO-16388) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cacna1f-KO
Strain ID
KOCMP-54652-Cacna1f-B6J-VB
Gene Name
Product ID
S-KO-16388
Gene Alias
A930034B14, Cav1.4, Sfc17, nerg1, nob2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr X
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000115726
NCBI RefSeq
NM_019582
Target Region
Exon 14~17
Size of Effective Region
~2.4 kb
Overview of Gene Research
CACNA1F encodes the α1F subunit of an L-type voltage-gated calcium channel, Cav1.4. In photoreceptor synaptic terminals, Cav1.4 channels mediate glutamate release and postsynaptic responses associated with visual signal transmission [1]. This gene is also involved in the function of skeletal muscle as the Cav1.4 complex encoded by it impacts muscle functions [3].
In a Cacna1f-KO mouse model of congenital stationary night blindness 2A (CSNB2A), transgenic expression of Cacna1f rescued some visual function and restored wild-type-like photoreceptor and synaptic morphology, demonstrating the importance of Cacna1f in maintaining normal retinal function [2]. In a Cacna1f mutation rat model (Cacna1f(CSNB2) rat), the Cacna1f mutation reduced the function of slow-twitch skeletal muscle and affected synapse-associated protein expression, potentially blocking signal transmission in synaptic connectivity of the retina and skeletal muscle [3].
In conclusion, Cacna1f is crucial for visual signal transmission in the retina and for the normal function of skeletal muscle. Studies using gene-knockout mouse and rat models have revealed its role in congenital stationary night blindness and in modulating skeletal muscle function, providing insights into potential gene-therapy strategies for related diseases [2,3].
References:
1. Dai, Xufeng, Pang, Shiyi, Wang, Jieping, Pang, Jijing, Chang, Bo. 2018. Photoreceptor degeneration in a new Cacna1f mutant mouse model. In Experimental eye research, 179, 106-114. doi:10.1016/j.exer.2018.11.010. https://pubmed.ncbi.nlm.nih.gov/30445045/
2. Waldner, Derek M, Ito, Kenichi, Chen, Li-Li, Stell, William K, Bech-Hansen, N Torben. 2020. Transgenic Expression of Cacna1f Rescues Vision and Retinal Morphology in a Mouse Model of Congenital Stationary Night Blindness 2A (CSNB2A). In Translational vision science & technology, 9, 19. doi:10.1167/tvst.9.11.19. https://pubmed.ncbi.nlm.nih.gov/33117610/
3. An, Jing, Zhang, Lei, Jiao, Bo, Yu, Zhibin, Zhang, Zuoming. 2015. Cacna1f gene decreased contractility of skeletal muscle in rat model with congenital stationary night blindness. In Gene, 562, 210-9. doi:10.1016/j.gene.2015.02.073. https://pubmed.ncbi.nlm.nih.gov/25748727/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.