C57BL/6JCya-Lypd8em1/Cya
Common Name
Lypd8-KO
Product ID
S-KO-16419
Backgroud
C57BL/6JCya
Strain ID
KOCMP-70163-Lypd8-B6J-VB
When using this mouse strain in a publication, please cite “Lypd8-KO Mouse (Catalog S-KO-16419) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Lypd8-KO
Strain ID
KOCMP-70163-Lypd8-B6J-VB
Gene Name
Product ID
S-KO-16419
Gene Alias
2210415F13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000108826
NCBI RefSeq
NM_027339
Target Region
Exon 4~5
Size of Effective Region
~4.0 kb
Overview of Gene Research
Ly6/Plaur domain-containing 8 (Lypd8), a highly glycosylated glycosylphosphatidylinositol-anchored protein, is selectively expressed on colonic enterocytes. It plays a crucial role in maintaining gut homeostasis by segregating commensal microbes from the intestinal epithelia. Lypd8 may be involved in innate immunity-related pathways against enteric bacterial pathogens [1,2].
Lypd8-deficient mice show rapid colonization of Citrobacter rodentium in the colon, leading to severe colitis with Th17-cell and neutrophil expansion in the lamina propria, indicating its role in early-phase defense against this pathogen [1]. Also, in Lypd8 -/- mice, flagellated bacteria invade the mucosal surface of the colon, and when dysbiosis is induced by a high-fat diet, they develop spontaneous colitis [3]. These KO mouse models reveal that Lypd8 is essential for preventing bacterial invasion and maintaining a healthy gut environment.
In conclusion, Lypd8 is vital for maintaining gut homeostasis by preventing the attachment and invasion of bacteria, especially flagellated ones, to colonic epithelia. The Lypd8 KO mouse models have been instrumental in highlighting its role in protecting against colitis and other related intestinal diseases, suggesting its potential as a therapeutic target for such conditions [1,2,3].
References:
1. Okumura, Ryu, Kodama, Toshio, Hsu, Chiao-Ching, Iida, Tetsuya, Takeda, Kiyoshi. 2019. Lypd8 inhibits attachment of pathogenic bacteria to colonic epithelia. In Mucosal immunology, 13, 75-85. doi:10.1038/s41385-019-0219-4. https://pubmed.ncbi.nlm.nih.gov/31659301/
2. Okumura, Ryu, Kurakawa, Takashi, Nakano, Takashi, Sano, Koichi, Takeda, Kiyoshi. 2016. Lypd8 promotes the segregation of flagellated microbiota and colonic epithelia. In Nature, 532, 117-21. doi:10.1038/nature17406. https://pubmed.ncbi.nlm.nih.gov/27027293/
3. Hsu, Chiao-Ching, Okumura, Ryu, Motooka, Daisuke, Iida, Tetsuya, Takeda, Kiyoshi. . Alleviation of colonic inflammation by Lypd8 in a mouse model of inflammatory bowel disease. In International immunology, 33, 359-372. doi:10.1093/intimm/dxab012. https://pubmed.ncbi.nlm.nih.gov/33822948/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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