C57BL/6JCya-Brix1em1/Cya
Common Name:
Brix1-KO
Product ID:
S-KO-16451
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Brix1-KO
Strain ID
KOCMP-67832-Brix1-B6J-VB
Gene Name
Product ID
S-KO-16451
Gene Alias
1110064N10Rik; Bxdc2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Brix1em1/Cya mice (Catalog S-KO-16451) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022855
NCBI RefSeq
NM_026396
Target Region
Exon 3~10
Size of Effective Region
~6.3 kb
Detailed Document
Overview of Gene Research
BRIX1, also known as biogenesis of ribosomes BRX1, is a nucleolar protein critically involved in ribosome biogenesis. It facilitates pre-rRNA processing by supporting the formation of the PeBoW complex and is part of pathways related to ribosome assembly and biogenesis [1,2]. BRIX1 is also important in maintaining the homeostasis between ribosome biogenesis and p53 activation, and its abnormal expression may be associated with multiple biological processes and diseases.
Depletion of BRIX1 in cancer cells has shown significant effects. It induces nucleolar stress, which in turn activates p53 through RPL5 and RPL11, leading to the inhibition of tumor growth. Engineered exosomes loaded with siRNAs specific to BRIX1 can suppress the growth of colorectal cancer and enhance the efficacy of 5-FU chemotherapy in vivo [1]. In colorectal cancer, BRIX1 knockdown decreases rRNA levels, nascent RNA synthesis, glycolysis, and cell proliferation, while overexpression has the opposite effects. It also promotes ribosome synthesis and enhances glycolysis by selected translation of GLUT1 [2]. Additionally, in colorectal cancer patients, higher BRIX1 expression is positively correlated with tumor stage and serves as a risk factor for overall survival [3].
In conclusion, BRIX1 plays a crucial role in ribosome biogenesis and is involved in the progression of cancer, especially colorectal cancer. The use of gene-targeting methods such as siRNA-loaded exosomes targeting BRIX1 in in vivo studies shows potential as a cancer treatment strategy, highlighting the importance of understanding BRIX1 function for cancer therapy.
References:
1. Gan, Yu, Hao, Qian, Han, Tao, Chen, Jiaxiang, Zhou, Xiang. 2024. Targeting BRIX1 via Engineered Exosomes Induces Nucleolar Stress to Suppress Cancer Progression. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2407370. doi:10.1002/advs.202407370. https://pubmed.ncbi.nlm.nih.gov/39475053/
2. Jiang, Chunhui, Sun, Longci, Wen, Siyuan, Xu, Qing, Xue, Hanbing. . BRIX1 promotes ribosome synthesis and enhances glycolysis by selected translation of GLUT1 in colorectal cancer. In The journal of gene medicine, 26, e3632. doi:10.1002/jgm.3632. https://pubmed.ncbi.nlm.nih.gov/38282151/
3. Ge, Jianxin, Huang, Xiaoli, Wang, Ping, Lu, Cuihua. . Expression of biogenesis of ribosomes BRX1 is associated with malignant progression and prognosis in colorectal cancer. In Translational cancer research, 9, 5595-5602. doi:10.21037/tcr-20-2564. https://pubmed.ncbi.nlm.nih.gov/35117923/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen