C57BL/6JCya-Mbd3l2em1/Cya
Common Name
Mbd3l2-KO
Product ID
S-KO-16517
Backgroud
C57BL/6JCya
Strain ID
KOCMP-234988-Mbd3l2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Mbd3l2-KO Mouse (Catalog S-KO-16517) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mbd3l2-KO
Strain ID
KOCMP-234988-Mbd3l2-B6J-VB
Gene Name
Product ID
S-KO-16517
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 9
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000213777
NCBI RefSeq
NM_144934
Target Region
Exon 3
Size of Effective Region
~3.3 kb
Overview of Gene Research
Mbd3l2, a protein-coding gene, is homologous to MBD2 and MBD3 but lacks the methyl-CpG-binding domain [2,4]. It is involved in epigenetic regulation and transcriptional modulation. Mbd3l2 can interact with MBD3 and components of the NuRD complex, and may play a role in processes related to DNA methylation-mediated gene silencing and activation [2].
Knockout mouse models of Mbd3l2 were created to study its function. Mbd3l2 knockout mice were viable and fertile, suggesting that Mbd3l2 is dispensable for early development, fertility, and zygotic DNA demethylation in the mouse [3].
In summary, while Mbd3l2-knockout mouse models indicate that it is not essential for early development and zygotic DNA demethylation, it still plays important roles in epigenetic regulation, such as modulating Tet2 enzymatic activity in 5-methylcytosine oxidation, and interacting with components of the NuRD complex to potentially reactivate transcription from methylated DNA [1,2]. These functions are relevant for understanding epigenetic-related disease mechanisms like malignancies [1].
References:
1. Peng, Lina, Li, Yan, Xi, Yanping, Wu, Feizhen, Yu, Wenqiang. 2016. MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation. In Journal of cell science, 129, 1059-71. doi:10.1242/jcs.179044. https://pubmed.ncbi.nlm.nih.gov/26769901/
2. Jin, Seung-Gi, Jiang, Chun-Ling, Rauch, Tibor, Li, Hongwei, Pfeifer, Gerd P. 2005. MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing. In The Journal of biological chemistry, 280, 12700-9. doi:. https://pubmed.ncbi.nlm.nih.gov/15701600/
3. Jin, Seung-Gi, Tsark, Walter, Szabó, Piroska E, Pfeifer, Gerd P. . Haploid male germ cell- and oocyte-specific Mbd3l1 and Mbd3l2 genes are dispensable for early development, fertility, and zygotic DNA demethylation in the mouse. In Developmental dynamics : an official publication of the American Association of Anatomists, 237, 3435-43. doi:10.1002/dvdy.21767. https://pubmed.ncbi.nlm.nih.gov/18942147/
4. Jiang, Chun Ling, Jin, Seung Gi, Lee, Dong Hyun, Cooney, Austin J, Pfeifer, Gerd P. . MBD3L1 and MBD3L2, two new proteins homologous to the methyl-CpG-binding proteins MBD2 and MBD3: characterization of MBD3L1 as a testis-specific transcriptional repressor. In Genomics, 80, 621-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12504854/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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