C57BL/6JCya-Gcem1/Cya
Common Name
Gc-KO
Product ID
S-KO-16525
Backgroud
C57BL/6JCya
Strain ID
KOCMP-14473-Gc-B6J-VB
When using this mouse strain in a publication, please cite “Gc-KO Mouse (Catalog S-KO-16525) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Gc-KO
Strain ID
KOCMP-14473-Gc-B6J-VB
Gene Name
Product ID
S-KO-16525
Gene Alias
DBP, VDB
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 5
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000049209
NCBI RefSeq
NM_008096
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Overview of Gene Research
There is some ambiguity as "Gc" can refer to different entities. One possible interpretation is that it might be related to genomic guanine-cytosine (GC) content. GC content is a fundamental component of genetic diversity, crucial for phylogenetic analyses [3]. In genomes, the ratio of GC base pairs to the total number of base pairs can influence various biological processes. GC pairs are generally more stable than AT pairs, and in prokaryotes, there is a positive correlation between growth temperature and GC content, suggesting thermal adaptation [2].
In gerbil genomes, a large genomic region with extreme GC-content has been discovered. Recombination, through GC-biased gene conversion (gBGC), increases the local GC-content in genomic regions. In gerbils, this high-GC region is characterized by high substitution rates for all mutational categories. Additionally, over 300 genes outside the known region have outlying values of AT-to-GC synonymous substitution rates, with over 30% organized into at least 17 large clusters. This suggests the evolution of genomic regions with very high recombination rates in the gerbil lineage, leading to a runaway increase in GC-content [1].
In conclusion, GC content, whether in terms of overall genomic GC levels or local GC-biased evolution, plays significant roles in various biological processes such as evolution, adaptation to environmental conditions like temperature, and potentially in the context of genomic stability and rearrangement. The study of gerbil genomes provides a powerful model to understand the mechanisms of gBGC and the rapid evolution of GC-content in mammals [1].
References:
1. Pracana, Rodrigo, Hargreaves, Adam D, Mulley, John F, Holland, Peter W H. . Runaway GC Evolution in Gerbil Genomes. In Molecular biology and evolution, 37, 2197-2210. doi:10.1093/molbev/msaa072. https://pubmed.ncbi.nlm.nih.gov/32170949/
2. Hu, En-Ze, Lan, Xin-Ran, Liu, Zhi-Ling, Gao, Jie, Niu, Deng-Ke. 2022. A positive correlation between GC content and growth temperature in prokaryotes. In BMC genomics, 23, 110. doi:10.1186/s12864-022-08353-7. https://pubmed.ncbi.nlm.nih.gov/35139824/
3. Xian, Qing, Wang, Suyin, Liu, Yanyan, Kan, Shenglong, Zhang, Wei. 2023. Structure-Based GC Investigation Sheds New Light on ITS2 Evolution in Corydalis Species. In International journal of molecular sciences, 24, . doi:10.3390/ijms24097716. https://pubmed.ncbi.nlm.nih.gov/37175423/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.