Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Mettl8em1/Cya
Common Name:
Mettl8-KO
Product ID:
S-KO-16539
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mettl8-KO
Strain ID
KOCMP-228019-Mettl8-B6J-VB
Gene Name
Mettl8
Product ID
S-KO-16539
Gene Alias
Tip
Background
C57BL/6JCya
NCBI ID
228019
Modification
Conventional knockout
Chromosome
2
Phenotype
MGI:2385142
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mettl8em1/Cya mice (Catalog S-KO-16539) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112186
NCBI RefSeq
NM_145524
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mettl8, or methyltransferase-like 8, is a gene encoding an enzyme involved in RNA modification. It is a key factor in the 3-methylcytosine (m3C) modification of mitochondrial tRNAs, which is crucial for mitochondrial protein translation, respiration, and overall cellular energy metabolism. The role of Mettl8 in biological systems can be studied using genetic models such as knockout mice [1,2,3].

In mouse embryonic cortical neural stem cells, Mettl8 is localized in mitochondria and installs m3C specifically on mitochondrial tRNAThr/Ser(UCN). Deletion of Mettl8 in these cells leads to reduced mitochondrial protein translation, attenuated respiration activity, and impaired embryonic cortical neural stem cell maintenance in vivo, which can be rescued by enhancing mitochondrial functions. Similarly, in human forebrain cortical organoids, METTL8 promotes mitochondrial protein expression and neural stem cell maintenance [1]. In pancreatic cancer, METTL8 levels are high, correlating with lower patient survival and enhanced respiratory chain activity. METTL8 knockout cells show a reduction in respiratory chain activity, while overexpression increases it. Mitochondrial ribosome profiling in knockout cells uncovered mitoribosome stalling on mt-tRNASer(UCN)-and mt-tRNAThr-dependent codons, and reduced incorporation of mitochondrially encoded proteins ND6 and ND1 into complex I [2]. In glioblastoma stem cells (GSCs), METTL8 is localized to the mitochondrial matrix, where it installs m3C on mt-tRNAThr/Ser(UCN) for mitochondrial translation and respiration. High METTL8 expression in glioblastoma is attributed to histone variant H2AZ-mediated chromatin accessibility of HIF1α and portends inferior patient outcome. Depletion of METTL8 impairs GSC self-renewal and differentiation, retarding tumor growth in an intracranial GBM xenograft model, and also inactivates the RTK/Akt axis, leading to heightened sensitivity to Akt inhibitor treatment [3].

In conclusion, Mettl8 is essential for the m3C modification of mitochondrial tRNAs, which is crucial for mitochondrial translation, respiration, and cell fate maintenance in processes like cortical neurogenesis. Its dysregulation is associated with diseases such as pancreatic cancer and glioblastoma. The use of Mettl8 knockout or conditional knockout mouse models has provided valuable insights into its role in these biological processes and disease conditions, helping to understand the underlying mechanisms and potentially paving the way for new therapeutic strategies.

References:

1. Zhang, Feng, Yoon, Kijun, Zhang, Daniel Y, Ming, Guo-Li, Song, Hongjun. 2023. Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m3C modification. In Cell stem cell, 30, 300-311.e11. doi:10.1016/j.stem.2023.01.007. https://pubmed.ncbi.nlm.nih.gov/36764294/

2. Schöller, Eva, Marks, James, Marchand, Virginie, Hafner, Markus, Meister, Gunter. 2021. Balancing of mitochondrial translation through METTL8-mediated m3C modification of mitochondrial tRNAs. In Molecular cell, 81, 4810-4825.e12. doi:10.1016/j.molcel.2021.10.018. https://pubmed.ncbi.nlm.nih.gov/34774131/

3. Lee, Bernice Woon Li, Chuah, You Heng, Yoon, Jeehyun, Lin, Zhewang, Ong, Derrick Sek Tong. 2024. METTL8 links mt-tRNA m3C modification to the HIF1α/RTK/Akt axis to sustain GBM stemness and tumorigenicity. In Cell death & disease, 15, 338. doi:10.1038/s41419-024-06718-2. https://pubmed.ncbi.nlm.nih.gov/38744809/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest