C57BL/6JCya-Fam163aem1/Cya
Common Name
Fam163a-KO
Product ID
S-KO-16554
Backgroud
C57BL/6JCya
Strain ID
KOCMP-329274-Fam163a-B6J-VB
When using this mouse strain in a publication, please cite “Fam163a-KO Mouse (Catalog S-KO-16554) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fam163a-KO
Strain ID
KOCMP-329274-Fam163a-B6J-VB
Gene Name
Product ID
S-KO-16554
Gene Alias
A230106N23Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000015628
NCBI RefSeq
NM_177838
Target Region
Exon 4~5
Size of Effective Region
~2.1 kb
Overview of Gene Research
Fam163a, also called neuroblastoma-derived secretory protein (NDSP) or C1ORF76, is located on chromosome 1q25.2. It has been implicated in multiple biological processes. In the ERK signaling pathway, it acts as a positive regulator, promoting cell proliferation. It also may be part of the mitochondrial proteome, potentially playing a role in mitochondrial-related functions [1,2].
In squamous cell lung carcinoma, Fam163a overexpression increases ERK and p90RSK phosphorylation, promotes cyclin D1 expression, and directly interacts with 14-3-3β and ERK, regulating ERK phosphorylation by upregulating 14-3-3β protein levels. Its expression correlates with larger tumor size, TNM staging, and regional lymph node metastasis, and patients with positive expression have shorter survival times [1]. In hypothalamic arcuate nucleus, Fam163a knockdown increases short-term food intake, reduces leptin levels, and decreases the firing frequency of AgRP neurons, while also differentially regulating mitochondrial-related gene expression [2].
In conclusion, Fam163a has important functions in cell proliferation and mitochondrial-related processes. Its role in promoting cell proliferation in squamous cell lung carcinoma and modulating AgRP neuron activity in the hypothalamus through mitochondrial-related mechanisms, as revealed by in vivo studies, indicates its significance in cancer and metabolism-related disease areas.
References:
1. Liu, Nan, Zhou, Haijing, Zhang, Xiupeng, Li, Ailin, Miao, Yuan. 2019. FAM163A, a positive regulator of ERK signaling pathway, interacts with 14-3-3β and promotes cell proliferation in squamous cell lung carcinoma. In OncoTargets and therapy, 12, 6393-6406. doi:10.2147/OTT.S214731. https://pubmed.ncbi.nlm.nih.gov/31496741/
2. Erdogan, Cihan Suleyman, Yavuz, Yavuz, Ozgun, Huseyin Bugra, Kalkan, Ugur Faruk, Yilmaz, Bayram. . Fam163a knockdown and mitochondrial stress in the arcuate nucleus of hypothalamus reduce AgRP neuron activity and differentially regulate mitochondrial dynamics in mice. In Acta physiologica (Oxford, England), 241, e70020. doi:10.1111/apha.70020. https://pubmed.ncbi.nlm.nih.gov/40071489/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.