C57BL/6JCya-Tbataem1/Cya
Common Name
Tbata-KO
Product ID
S-KO-16634
Backgroud
C57BL/6JCya
Strain ID
KOCMP-65971-Tbata-B6J-VB
Status
When using this mouse strain in a publication, please cite “Tbata-KO Mouse (Catalog S-KO-16634) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Tbata-KO
Strain ID
KOCMP-65971-Tbata-B6J-VB
Gene Name
Product ID
S-KO-16634
Gene Alias
Titest, Spatial, 1700021K02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000035894
NCBI RefSeq
NM_001017433
Target Region
Exon 4~5
Size of Effective Region
~1.1 kb
Overview of Gene Research
Tbata, also known as SPATIAL, is a gene that plays crucial roles in multiple biological processes. It is expressed in thymic stromal cells, brain neurons, and testis germ cells. In thymic stromal cells, Tbata regulates thymic epithelial cell (TEC) proliferation and thymus size by interacting with the enzyme Uba3 and inhibiting the Nedd8 pathway [1]. In neurons, it is involved in neurite outgrowth and dendrite patterning, with its expression being increased by Nerve Growth Factor (NGF) and its localization to dendrites relying on the kinesin motor Kif17 [2].
In gene-related studies, thymi from aged Tbata-deficient mice are larger and contain more dividing TECs compared to wild-type littermate controls. Also, thymic reconstitution after bone marrow transplantation occurs more rapidly in Rag2(-/-)Tbata(-/-) mice than in Rag2(-/-)Tbata(+/+) littermate controls, suggesting its role in thymus function regulation via stromal cell proliferation [1]. In zebrafish, knockdown of Tbata orthologs shows its indispensability for enteric nervous system (ENS) development, and this was confirmed by CRISPR knockout, indicating its role in ENS-related processes [3].
In conclusion, Tbata is essential for thymus function through its regulation of stromal cell proliferation and also plays important roles in neuronal development and ENS development. The use of gene-knockout mouse models and zebrafish models has provided significant insights into Tbata's functions in these biological processes.
References:
1. Flomerfelt, Francis A, El Kassar, Nahed, Gurunathan, Chandra, Schwartz, Ronald H, Gress, Ronald E. 2010. Tbata modulates thymic stromal cell proliferation and thymus function. In The Journal of experimental medicine, 207, 2521-32. doi:10.1084/jem.20092759. https://pubmed.ncbi.nlm.nih.gov/20937703/
2. Yammine, Miriam, Saade, Murielle, Chauvet, Sophie, Nguyen, Catherine. 2013. Spatial gene's (Tbata) implication in neurite outgrowth and dendrite patterning in hippocampal neurons. In Molecular and cellular neurosciences, 59, 1-9. doi:10.1016/j.mcn.2013.12.007. https://pubmed.ncbi.nlm.nih.gov/24361585/
3. Gui, Hongsheng, Schriemer, Duco, Cheng, William W, Garcia-Barceló, Maria-Mercè, Hofstra, Robert M W. 2017. Whole exome sequencing coupled with unbiased functional analysis reveals new Hirschsprung disease genes. In Genome biology, 18, 48. doi:10.1186/s13059-017-1174-6. https://pubmed.ncbi.nlm.nih.gov/28274275/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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