C57BL/6JCya-Lsm11em1/Cya
Common Name:
Lsm11-KO
Product ID:
S-KO-16727
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lsm11-KO
Strain ID
KOCMP-72290-Lsm11-B6J-VB
Gene Name
Product ID
S-KO-16727
Gene Alias
2210404M20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lsm11em1/Cya mice (Catalog S-KO-16727) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000129820
NCBI RefSeq
NM_028185
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Lsm11, a component of the replication-dependent histone pre-mRNA-processing complex, is crucial for the 3' end processing of metazoan replication-dependent histone pre-mRNAs. U7 snRNP, which contains Lsm11 and Lsm10 (replacing SmD1 and SmD2 in the canonical heptameric Sm protein ring), is essential for this process. Lsm11 is also involved in pathways related to the assembly of U7 snRNP, as it tightly interacts with the methylosome composed of PRMT5, MEP50, and pICln, which methylates Lsm11 [2,3].
Mutations in Lsm11 have been associated with Aicardi-Goutières syndrome, a rare monogenic autoimmune disease. These mutations lead to misprocessing of canonical histone transcripts, disturbance of linker histone stoichiometry, and enhanced interferon signaling mediated by the cGAS-STING pathway in patient-derived fibroblasts [1]. In Drosophila, a mutation in Lsm11 that prevents the binding of FLASH (a processing factor that binds Lsm11) cannot rescue the lethality or histone pre-mRNA processing defects resulting from an Lsm11 null mutation, demonstrating the essential role of the dFLASH-dLsm11 interaction for histone pre-mRNA processing in vivo and proper development in flies [4].
In conclusion, Lsm11 is essential for histone pre-mRNA processing and plays a significant role in development. Its malfunction due to mutations is associated with Aicardi-Goutières syndrome. Studies in model organisms like Drosophila have revealed its key interactions and functions, providing insights into the biological processes it is involved in and the disease mechanisms related to its dysfunction.
References:
1. Uggenti, Carolina, Lepelley, Alice, Depp, Marine, Gilbert, Nick, Crow, Yanick J. 2020. cGAS-mediated induction of type I interferon due to inborn errors of histone pre-mRNA processing. In Nature genetics, 52, 1364-1372. doi:10.1038/s41588-020-00737-3. https://pubmed.ncbi.nlm.nih.gov/33230297/
2. Yang, Xiao-Cui, Desotell, Anthony, Lin, Min-Han, Tong, Liang, Dominski, Zbigniew. 2023. In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome. In RNA (New York, N.Y.), 29, 1673-1690. doi:10.1261/rna.079709.123. https://pubmed.ncbi.nlm.nih.gov/37562960/
3. Yang, Xiao-Cui, Desotell, Anthony, Lin, Min-Han, Tong, Liang, Dominski, Zbigniew. 2023. In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.10.540203. https://pubmed.ncbi.nlm.nih.gov/37215023/
4. Burch, Brandon D, Godfrey, Ashley C, Gasdaska, Pamela Y, Marzluff, William F, Dominski, Zbigniew. 2011. Interaction between FLASH and Lsm11 is essential for histone pre-mRNA processing in vivo in Drosophila. In RNA (New York, N.Y.), 17, 1132-47. doi:10.1261/rna.2566811. https://pubmed.ncbi.nlm.nih.gov/21525146/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen