C57BL/6JCya-Mgst3em1/Cya
Common Name
Mgst3-KO
Product ID
S-KO-16733
Backgroud
C57BL/6JCya
Strain ID
KOCMP-66447-Mgst3-B6J-VB
Status
When using this mouse strain in a publication, please cite “Mgst3-KO Mouse (Catalog S-KO-16733) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mgst3-KO
Strain ID
KOCMP-66447-Mgst3-B6J-VB
Gene Name
Product ID
S-KO-16733
Gene Alias
GST-III, 2010012L10Rik, 2010306B17Rik, 2700004G04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000028005
NCBI RefSeq
NM_025569
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Overview of Gene Research
Mgst3, or microsomal glutathione transferase 3, is part of the antioxidant system and is involved in eicosanoid and glutathione metabolism [1,2]. These processes are associated with oxidative stress, apoptosis, and may play a role in the pathophysiology of neurodegenerative and other diseases [1,2]. Genetic models, such as gene knockout mouse models, can be valuable in studying Mgst3's functions.
In neurodegenerative disease-related studies, silencing Mgst3 downregulates the interaction between UBL3 and α-synuclein, which are associated with the pathology of Parkinson's disease [1]. In Alzheimer's disease research, knockdown of Mgst3 in cell lines reduces the protein level of beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) and amyloidogenesis, through a mechanism involving RGS4-mediated AKT signaling pathway [2]. In triple-negative breast cancer, mutant p53 protects cells from ferroptosis via NRF2-dependent regulation of Mgst3, encoding a glutathione-dependent peroxidase that detoxifies lipid peroxides [3].
In conclusion, Mgst3 is essential in antioxidant-related processes and is implicated in multiple disease areas including neurodegenerative diseases like Parkinson's and Alzheimer's, as well as in triple-negative breast cancer. Studies using loss-of-function models have been crucial in revealing its role in these diseases, providing insights into potential disease mechanisms and possible therapeutic targets.
References:
1. Yan, Jing, Zhang, Hengsen, Tomochika, Yuna, Yoshida, Minoru, Setou, Mitsutoshi. 2023. UBL3 Interaction with α-Synuclein Is Downregulated by Silencing MGST3. In Biomedicines, 11, . doi:10.3390/biomedicines11092491. https://pubmed.ncbi.nlm.nih.gov/37760932/
2. Pu, Yalan, Yang, Jie, Pan, Qiuling, Xiao, Fei, Chen, Guojun. 2024. MGST3 regulates BACE1 protein translation and amyloidogenesis by controlling the RGS4-mediated AKT signaling pathway. In The Journal of biological chemistry, 300, 107530. doi:10.1016/j.jbc.2024.107530. https://pubmed.ncbi.nlm.nih.gov/38971310/
3. Dibra, Denada, Xiong, Shunbin, Moyer, Sydney M, Korkut, Anil, Lozano, Guillermina. 2024. Mutant p53 protects triple-negative breast adenocarcinomas from ferroptosis in vivo. In Science advances, 10, eadk1835. doi:10.1126/sciadv.adk1835. https://pubmed.ncbi.nlm.nih.gov/38354236/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.