C57BL/6JCya-Liphem1/Cya
Common Name:
Liph-KO
Product ID:
S-KO-16737
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Liph-KO
Strain ID
KOCMP-239759-Liph-B6J-VB
Gene Name
Product ID
S-KO-16737
Gene Alias
C130037N08Rik; D16Wsu119e; H06; Lpdlr; P3; PLA1B; lpd1; lpd2; mPA-PLA1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Liphem1/Cya mice (Catalog S-KO-16737) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060673
NCBI RefSeq
NM_001083894
Target Region
Exon 3
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
LIPH, a member of the triglyceride lipase family, is a membrane-associated phosphatidic acid-selective phospholipase A1a. It can generate LPA from PA on the plasma membrane outer leaflet. The LIPH-LPA-LPAR6 signaling pathway is important, and LIPH dysfunction is linked to lipid metabolism disorders [1,8].
In pancreatic ductal adenocarcinoma (PDAC), LIPH is upregulated. Its down-regulation inhibits PDAC cell colony formation and proliferation. LIPH promotes tumor glycolysis by activating the LPA/LPAR axis, triggering the PI3K/AKT/HIF1A signaling pathway, and maintaining ALDOA stability in the cytosol. PDAC with high LIPH expression is sensitive to gemcitabine-based therapy [1].
In triple-negative breast cancer (TNBC), LIPH up-regulation promotes metastasis and stemness of TNBC cells. LIPH knockdown reduces the ratio and mammosphere-forming ability of CD44 + /CD24-stem-like cells, promotes apoptosis, arrests the cell cycle, and reduces metabolism [2].
In breast cancer, both mRNA and protein expression of LIPH are upregulated, and high LIPH expression is related to a lower overall survival rate [3].
In pancreatic cancer, LIPH overexpression is associated with tumor recurrence, advanced histologic grade, and poorer overall survival, and is correlated with immune suppression or evasion [5].
In esophageal squamous cell carcinoma (ESCC), a novel lncRNA LIPH-4 promotes ESCC progression through the miR-216b/IGF2BP2 axis [6].
Mutations in LIPH are responsible for autosomal recessive woolly hair/hypotrichosis in humans and the rex short-hair phenotype in rabbits [4,7,8].
In conclusion, LIPH is involved in multiple biological processes and disease conditions. Functional studies, especially those related to loss-of-function in relevant models (implied by the cell-based and patient-derived studies), have revealed its role in cancer progression, metastasis, metabolism, and hair-related disorders. Understanding LIPH can provide insights into disease mechanisms and potential therapeutic targets for cancers like PDAC, TNBC, breast cancer, pancreatic cancer, ESCC, as well as hair-related genetic diseases.
References:
1. Han, Lijie, Jiang, Yongsheng, Shi, Minmin, Jiang, Lingxi, Chen, Hao. 2023. LIPH contributes to glycolytic phenotype in pancreatic ductal adenocarcinoma by activating LPA/LPAR axis and maintaining ALDOA stability. In Journal of translational medicine, 21, 838. doi:10.1186/s12967-023-04702-6. https://pubmed.ncbi.nlm.nih.gov/37990271/
2. Zhang, Yixiao, Zhu, Xudong, Qiao, Xinbo, Cui, Meizi, Liu, Caigang. 2020. LIPH promotes metastasis by enriching stem-like cells in triple-negative breast cancer. In Journal of cellular and molecular medicine, 24, 9125-9134. doi:10.1111/jcmm.15549. https://pubmed.ncbi.nlm.nih.gov/32618099/
3. Gao, Peng, Liu, Qiang, Ai, Bolun, Wang, Zhongzhao, Wang, Jing. 2021. Prognostic Value and Clinical Significance of LIPH in Breast Cancer. In International journal of general medicine, 14, 7613-7623. doi:10.2147/IJGM.S332233. https://pubmed.ncbi.nlm.nih.gov/34754232/
4. Mizukami, Yukari, Hayashi, Ryota, Tsuruta, Daisuke, Shimomura, Yutaka, Sugawara, Koji. 2018. Novel splice site mutation in the LIPH gene in a patient with autosomal recessive woolly hair/hypotrichosis: Case report and published work review. In The Journal of dermatology, 45, 613-617. doi:10.1111/1346-8138.14257. https://pubmed.ncbi.nlm.nih.gov/29464811/
5. Zhuang, Hongkai, Chen, Xinming, Wang, Ying, Zhang, Chuanzhao, Hou, Baohua. 2021. Identification of LIPH as an unfavorable biomarkers correlated with immune suppression or evasion in pancreatic cancer based on RNA-seq. In Cancer immunology, immunotherapy : CII, 71, 601-612. doi:10.1007/s00262-021-03019-x. https://pubmed.ncbi.nlm.nih.gov/34279685/
6. Xiao, Yuhang, Tang, Jinming, Yang, Desong, Wang, Wenxiang, Su, Min. 2022. Long noncoding RNA LIPH-4 promotes esophageal squamous cell carcinoma progression by regulating the miR-216b/IGF2BP2 axis. In Biomarker research, 10, 60. doi:10.1186/s40364-022-00408-x. https://pubmed.ncbi.nlm.nih.gov/35971159/
7. Diribarne, Mathieu, Mata, Xavier, Rivière, Julie, Cribiu, Edmond-Paul, Guérin, Gérard. 2012. LIPH expression in skin and hair follicles of normal coat and Rex rabbits. In PloS one, 7, e30073. doi:10.1371/journal.pone.0030073. https://pubmed.ncbi.nlm.nih.gov/22272275/
8. Akiyama, M. 2021. Isolated autosomal recessive woolly hair/hypotrichosis: genetics, pathogenesis and therapies. In Journal of the European Academy of Dermatology and Venereology : JEADV, 35, 1788-1796. doi:10.1111/jdv.17350. https://pubmed.ncbi.nlm.nih.gov/33988877/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen