C57BL/6JCya-Slc10a2em1/Cya
Common Name
Slc10a2-KO
Product ID
S-KO-16831
Backgroud
C57BL/6JCya
Strain ID
KOCMP-20494-Slc10a2-B6J-VB
When using this mouse strain in a publication, please cite “Slc10a2-KO Mouse (Catalog S-KO-16831) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Slc10a2-KO
Strain ID
KOCMP-20494-Slc10a2-B6J-VB
Gene Name
Product ID
S-KO-16831
Gene Alias
9130221J18Rik, ASBT, IBAT, ISBT
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000023835
NCBI RefSeq
NM_011388
Target Region
Exon 4
Size of Effective Region
~2.4 kb
Overview of Gene Research
Slc10a2, also known as the apical sodium-dependent bile acid transporter (ASBT), is a key protein in the solute carrier family. It is mainly expressed in the small intestine and is crucial for the reabsorption of ileal bile acids. This function is central to the enterohepatic circulation of bile acids and cholesterol homeostasis, which is vital for fat digestion [1,3].
In Slc10a2-null mice, fecal bile acid excretion is augmented more than 10-fold. These mice show a 54% increase in aberrant crypt foci, and 70% and 59% increases in colon tumor number and size respectively, indicating that endogenous fecal bile acids promoted by Slc10a2 deficiency may play a role in colon carcinogenesis [4]. Also, a human case with a homozygous mutation of SLC10A2 presented chronic bile acid diarrhea and severe stunting, expanding the mutant spectrum of the gene [2].
In conclusion, Slc10a2 is essential for maintaining bile acid and cholesterol balance through its role in the enterohepatic circulation. The study of Slc10a2-null mouse models and human genetic mutants has provided insights into its role in diseases such as colon cancer and bile acid-related diarrhea, highlighting its potential as a therapeutic target for these conditions.
References:
1. Yang, Na, Dong, Ya-Qian, Jia, Guo-Xiang, Yang, Shen-Shen, Li, Yu-Bo. 2020. ASBT(SLC10A2): A promising target for treatment of diseases and drug discovery. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 132, 110835. doi:10.1016/j.biopha.2020.110835. https://pubmed.ncbi.nlm.nih.gov/33035828/
2. Qie, Di, Zhang, Yulin, Gong, Xue, Lu, Guoyan, Li, Yifei. 2021. SLC10A2 deficiency-induced congenital chronic bile acid diarrhea and stunting. In Molecular genetics & genomic medicine, 9, e1740. doi:10.1002/mgg3.1740. https://pubmed.ncbi.nlm.nih.gov/34192422/
3. Xiao, Ling, Pan, Guoyu. 2017. An important intestinal transporter that regulates the enterohepatic circulation of bile acids and cholesterol homeostasis: The apical sodium-dependent bile acid transporter (SLC10A2/ASBT). In Clinics and research in hepatology and gastroenterology, 41, 509-515. doi:10.1016/j.clinre.2017.02.001. https://pubmed.ncbi.nlm.nih.gov/28336180/
4. Raufman, Jean-Pierre, Dawson, Paul A, Rao, Anuradha, Polli, James E, Cheng, Kunrong. 2015. Slc10a2-null mice uncover colon cancer-promoting actions of endogenous fecal bile acids. In Carcinogenesis, 36, 1193-200. doi:10.1093/carcin/bgv107. https://pubmed.ncbi.nlm.nih.gov/26210740/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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