C57BL/6JCya-Trpm5em1/Cya
Common Name:
Trpm5-KO
Product ID:
S-KO-17109
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Trpm5-KO
Strain ID
KOCMP-56843-Trpm5-B6J-VB
Gene Name
Product ID
S-KO-17109
Gene Alias
9430099A16Rik; LTrpC-5; Ltrpc5; Mtr1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trpm5em1/Cya mice (Catalog S-KO-17109) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000009390
NCBI RefSeq
NM_020277
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Trpm5, a Ca(2+)-activated cation channel, is crucial in taste and other chemosensory signaling [1,2,4]. In taste cells, it's the final element in a cascade triggered by the activation of G protein-coupled receptors for bitter, sweet, or umami tastes, relying on PLCβ2 to break down PIP2 and release Ca(2+) to activate Trpm5 [1,4]. It's distributed in chemosensory cells across the digestive, respiratory, and olfactory systems, and in pancreatic islets, contributing to insulin secretion [1]. Genetic models, like knockout mice, are valuable for studying its functions.
Trpm5-/-mice lack type II taste perception and show reduced glucose-induced insulin secretion, highlighting its role in taste transduction and pancreatic β-cell function [2]. In high-fat-diet mice, TRPM5 expression in pancreatic islets decreases, and in vitro studies with palmitate show lipotoxicity inhibits TRPM5 expression in pancreatic β-cells, driving β-cell dysfunction [5]. In B cells, Trpm5-deficient mice exhibit enhanced calcium-dependent responses to lipopolysaccharide, leading to increased proliferation and cytokine production, and exacerbation of endotoxic shock [6].
In conclusion, Trpm5 is essential for taste transduction and insulin secretion. Gene knockout mouse models have revealed its role in taste perception, pancreatic β-cell function, and immune responses such as in B cells during lipopolysaccharide stimulation. Understanding Trpm5 functions through these models may provide insights into diseases related to taste disorders, diabetes, and immune dysregulation.
References:
1. Liman, Emily R. . TRPM5. In Handbook of experimental pharmacology, 222, 489-502. doi:10.1007/978-3-642-54215-2_19. https://pubmed.ncbi.nlm.nih.gov/24756718/
2. Vennekens, R, Mesuere, M, Philippaert, K. 2017. TRPM5 in the battle against diabetes and obesity. In Acta physiologica (Oxford, England), 222, . doi:10.1111/apha.12949. https://pubmed.ncbi.nlm.nih.gov/28834354/
3. Uchida, Kunitoshi. 2024. TRPM3, TRPM4, and TRPM5 as thermo-sensitive channels. In The journal of physiological sciences : JPS, 74, 43. doi:10.1186/s12576-024-00937-0. https://pubmed.ncbi.nlm.nih.gov/39294615/
4. Liman, E R. . TRPM5 and taste transduction. In Handbook of experimental pharmacology, , 287-98. doi:. https://pubmed.ncbi.nlm.nih.gov/17217064/
5. Wang, Kai-Yuan, Wu, Shi-Mei, Yao, Zheng-Jian, Zhu, Yun-Xia, Han, Xiao. 2024. Insufficient TRPM5 Mediates Lipotoxicity-induced Pancreatic β-cell Dysfunction. In Current medical science, 44, 346-354. doi:10.1007/s11596-023-2795-5. https://pubmed.ncbi.nlm.nih.gov/38517672/
6. Sakaguchi, Taiki, Okumura, Ryu, Ono, Chisato, Baba, Yoshihiro, Takeda, Kiyoshi. . TRPM5 Negatively Regulates Calcium-Dependent Responses in Lipopolysaccharide-Stimulated B Lymphocytes. In Cell reports, 31, 107755. doi:10.1016/j.celrep.2020.107755. https://pubmed.ncbi.nlm.nih.gov/32521253/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen