Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Zcrb1em1/Cya
Common Name:
Zcrb1-KO
Product ID:
S-KO-17209
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Zcrb1-KO
Strain ID
KOCMP-67197-Zcrb1-B6J-VB
Gene Name
Zcrb1
Product ID
S-KO-17209
Gene Alias
2700088M22Rik; Madp-1
Background
C57BL/6JCya
NCBI ID
67197
Modification
Conventional knockout
Chromosome
15
Phenotype
MGI:1914447
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zcrb1em1/Cya mice (Catalog S-KO-17209) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000076070
NCBI RefSeq
NM_026025.3
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Zcrb1, also known as zinc finger CCHC-type and RNA-binding motif 1 (alias MADP-1), is a core component of the U12 mono-snRNP minor spliceosome [3]. It plays a role in regulating gene expression by influencing the splicing of U12-type genes. It is associated with processes like ciliogenesis and WNT signaling, and is conserved across species from zebrafish to human [1,3]. Genetic models, such as knockdown in zebrafish embryos and human cell lines, are valuable for studying its function.

In human cell lines, partial reduction of ZCRB1 expression via CRISPR-Cas9 and siRNA leads to dysregulation of U12-type gene splicing and expression, mainly due to U12 mono-snRNA dysregulation. This results in down-regulation of ciliogenesis-related genes and up-regulation of WNT signaling [1]. In zebrafish embryos, zcrb1 CRISPR-Cas12a knockdown causes gross developmental and body axis abnormalities, disrupted ciliogenesis, and up-regulated WNT signaling, complementing the human cell studies [1]. In glioblastoma multiforme (GBM), overexpression of ZCRB1 promotes the formation of circHEATR5B, which encodes a protein that suppresses aerobic glycolysis and proliferation in GBM cells [2]. In head and neck squamous cell carcinoma (HNSCC), knockdown of ZCRB1 impairs cell survival, while overexpression promotes tumor cellular proliferation, and its depletion contributes to aberrant splicing events in DNA damage response [4].

In summary, Zcrb1 is crucial for normal cellular and developmental processes, mainly through its role in minor spliceosome-mediated gene splicing. Its dysregulation is associated with various diseases, including GBM and HNSCC. The use of gene-knockdown models in zebrafish and human cell lines has been instrumental in uncovering these associations, providing insights into potential disease mechanisms and therapeutic targets.

References:

1. Powell-Rodgers, Geralle, Pirzada, Mujeeb Ur Rehman, Richee, Jahmiera, Stratman, Amber N, Djuranovic, Sergej. 2024. Role of U11/U12 minor spliceosome gene ZCRB1 in Ciliogenesis and WNT Signaling. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.09.607392. https://pubmed.ncbi.nlm.nih.gov/39149385/

2. Song, Jian, Zheng, Jian, Liu, Xiaobai, Zhang, Mengyang, Liu, Yunhui. 2022. A novel protein encoded by ZCRB1-induced circHEATR5B suppresses aerobic glycolysis of GBM through phosphorylation of JMJD5. In Journal of experimental & clinical cancer research : CR, 41, 171. doi:10.1186/s13046-022-02374-6. https://pubmed.ncbi.nlm.nih.gov/35538499/

3. Wang, Haoran, Gao, Mary X, Li, Linda, Hori, Naohiro, Sato, Kenzo. 2006. Isolation, expression, and characterization of the human ZCRB1 gene mapped to 12q12. In Genomics, 89, 59-69. doi:. https://pubmed.ncbi.nlm.nih.gov/16959469/

4. Chen, Chaoqun, Huang, Fang, Li, Xiaojie, Qi, Yangfan, Wang, Yang. 2024. Identification of splicing factors signature predicting prognosis risk and the mechanistic roles of novel oncogenes in HNSCC. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167115. doi:10.1016/j.bbadis.2024.167115. https://pubmed.ncbi.nlm.nih.gov/38458543/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest