Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Atad1em1/Cya
Common Name:
Atad1-KO
Product ID:
S-KO-17249
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Atad1-KO
Strain ID
KOCMP-67979-Atad1-B6J-VA
Gene Name
Atad1
Product ID
S-KO-17249
Gene Alias
4921525H23Rik; Thorase
Background
C57BL/6JCya
NCBI ID
67979
Modification
Conventional knockout
Chromosome
19
Phenotype
MGI:1915229
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atad1em1/Cya mice (Catalog S-KO-17249) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000235412
NCBI RefSeq
NM_026487.3
Target Region
Exon 5~6
Size of Effective Region
~2849 bp
Detailed Document
Click here to download >>
Overview of Gene Research
ATAD1, also known as ATPase family AAA domain-containing 1, is a crucial protein in maintaining mitochondrial homeostasis. It belongs to the AAA protein family and, like its yeast ortholog Msp1, uses ATP hydrolysis to recognize and extract mislocalized membrane proteins from the outer mitochondrial membrane. This function is essential for mitochondrial protein quality control, preventing clogging of the mitochondrial translocase of the outer membrane (TOM) and allowing for the proper import of nuclear-encoded proteins into mitochondria. ATAD1 is also involved in regulating synaptic plasticity, mitochondrial fission-fusion dynamics, and the degradation of desmin intermediate filaments in muscle [1,2,4,6].

In human cells, knockout of ATAD1 leads to extensive accumulation of mitochondrial precursors, decreased protein import, and clogging of TOM, demonstrating its importance in mitochondrial protein import surveillance [2]. In neurons, ATAD1 deficiency disrupts the mitochondrial fission-fusion balance, causing mitochondrial fragmentation, and impairs dendritic branching, spine maturation, and glutamatergic synaptic transmission. Rescue experiments with an ATP hydrolysis-deficient mutant showed that synaptic deficits rely on ATAD1's ATP hydrolysis activity [5]. In hepatitis C virus (HCV) infection, ATAD1 knockout significantly enhances HCV infection, as ATAD1 normally interacts with the viral TA-protein NS5B and induces its proteasomal degradation [3].

In conclusion, ATAD1 is vital for maintaining mitochondrial function, regulating neurodevelopment, synaptic function, and antiviral defense. The use of gene knockout models, such as in human cells and neurons, has revealed its role in specific biological processes and disease-related conditions, providing insights into the underlying mechanisms and potential therapeutic targets for mitochondrial-related disorders, neurodegenerative diseases, and HCV-related pathologies.

References:

1. Wang, Lan, Walter, Peter. 2020. Msp1/ATAD1 in Protein Quality Control and Regulation of Synaptic Activities. In Annual review of cell and developmental biology, 36, 141-164. doi:10.1146/annurev-cellbio-031220-015840. https://pubmed.ncbi.nlm.nih.gov/32886535/

2. Kim, John, Goldstein, Madeleine, Zecchel, Lauren, Maxwell, Christopher A, Weidberg, Hilla. 2024. ATAD1 prevents clogging of TOM and damage caused by un-imported mitochondrial proteins. In Cell reports, 43, 114473. doi:10.1016/j.celrep.2024.114473. https://pubmed.ncbi.nlm.nih.gov/39024102/

3. Zhou, Qing, Yang, Yuhao, Xu, Zhanxue, Gao, Song, Li, Yi-Ping. 2023. ATAD1 inhibits hepatitis C virus infection by removing the viral TA-protein NS5B from mitochondria. In EMBO reports, 24, e56614. doi:10.15252/embr.202256614. https://pubmed.ncbi.nlm.nih.gov/37789674/

4. He, Jiajia, Liu, Ke, Wu, Yifan, Tang, Ai-Hui, Fu, Chuanhai. 2023. The AAA-ATPase Yta4/ATAD1 interacts with the mitochondrial divisome to inhibit mitochondrial fission. In PLoS biology, 21, e3002247. doi:10.1371/journal.pbio.3002247. https://pubmed.ncbi.nlm.nih.gov/37590302/

5. Yan, Hao-Hao, He, Jia-Jia, Fu, Chuanhai, Chen, Jia-Hui, Tang, Ai-Hui. 2024. ATAD1 Regulates Neuronal Development and Synapse Formation Through Tuning Mitochondrial Function. In International journal of molecular sciences, 26, . doi:10.3390/ijms26010044. https://pubmed.ncbi.nlm.nih.gov/39795902/

6. Aweida, Dina, Cohen, Shenhav. 2022. The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in muscle. In EMBO reports, 23, e55175. doi:10.15252/embr.202255175. https://pubmed.ncbi.nlm.nih.gov/36278411/

Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest