C57BL/6JCya-Krt77em1/Cya
Common Name:
Krt77-KO
Product ID:
S-KO-17270
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Krt77-KO
Strain ID
KOCMP-406220-Krt77-B6J-VA
Gene Name
Product ID
S-KO-17270
Gene Alias
4732484G22Rik; CK-1B; K1emb; K77; Krt239; mK1b
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Krt77em1/Cya mice (Catalog S-KO-17270) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087996
NCBI RefSeq
NM_001003667.2
Target Region
Exon 2
Size of Effective Region
~215 bp
Detailed Document
Overview of Gene Research
Krt77, a type II epithelial cell α-keratin gene family member, plays a crucial role in animal epidermal and coat formation [1]. It is involved in epidermal development and is associated with processes like keratinocyte differentiation, skin development, and epithelial cell differentiation [2].
In a study on Chinese cattle, a 30-bp insertion in the Krt77 gene was found to be associated with environmental adaptation, with the insertion frequency increasing from the northwest to the southeast population and correlated with local annual mean temperature, relative humidity, and temperature humidity index [1]. In murine studies, the loss of Krt77 did not cause fetal lethality or harm skin and appendage development, but led to an up-regulation of Krt1, suggesting functional compensation [4]. In addition, Krt77 was identified as one of the key genes in the protein-protein interaction network analysis related to wool fiber diameter variation in Angora rabbits [2]. Also, its expression levels decreased from admission to discharge in COVID-19 patients, being related to the regulation of inflammation drivers in the disease [3].
In conclusion, Krt77 is essential for epidermal and coat formation. Studies on Krt77, especially through gene-knockout models in mice, have provided insights into its role in environmental adaptation in cattle, functional compensation in murine skin, wool fiber diameter determination in rabbits, and inflammation regulation in COVID-19. These findings contribute to understanding its biological functions and its implications in different biological processes and disease conditions.
References:
1. Zhang, Mengqi, Wu, Daoyi, Ahmed, Zulfiqar, Huang, Bizhi, Lei, Chuzhao. 2023. The genetic secrets of adaptation: decoding the significance of the 30-bp insertion in the KRT77 gene for Chinese cattle. In Animal biotechnology, 34, 3847-3854. doi:10.1080/10495398.2023.2232663. https://pubmed.ncbi.nlm.nih.gov/37452660/
2. Huang, Dongwei, Ding, Haisheng, Wang, Yuanlang, Wang, Xiaofei, Zhao, Huiling. 2024. Integration Analysis of Hair Follicle Transcriptome and Proteome Reveals the Mechanisms Regulating Wool Fiber Diameter in Angora Rabbits. In International journal of molecular sciences, 25, . doi:10.3390/ijms25063260. https://pubmed.ncbi.nlm.nih.gov/38542234/
3. Gade, Inger Lise, Bodilsen, Jacob, Mariager, Theis, Bennike, Tue Bjerg, Honoré, Bent. 2025. Exhaled breath protein composition in patients hospitalized during the first wave of COVID-19. In Journal of breath research, , . doi:10.1088/1752-7163/add617. https://pubmed.ncbi.nlm.nih.gov/40341493/
4. Ghorbanalipoor, S, Hommel, T, Kolbe, T, Posch, C, Dahlhoff, M. 2024. The loss of keratin 77 in murine skin is functionally compensated by keratin 1. In Biochimica et biophysica acta. Molecular cell research, 1872, 119881. doi:10.1016/j.bbamcr.2024.119881. https://pubmed.ncbi.nlm.nih.gov/39603462/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen