C57BL/6JCya-Arid5bem1/Cya
Common Name
Arid5b-KO
Product ID
S-KO-17286
Backgroud
C57BL/6JCya
Strain ID
KOCMP-71371-Arid5b-B6J-VA
When using this mouse strain in a publication, please cite “Arid5b-KO Mouse (Catalog S-KO-17286) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Arid5b-KO
Strain ID
KOCMP-71371-Arid5b-B6J-VA
Gene Name
Product ID
S-KO-17286
Gene Alias
4930580B11, 5430435G07Rik, Desrt, Mrf2, Mrf2alpha, Mrf2beta
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000219238
NCBI RefSeq
NM_023598.2
Target Region
Exon 6
Size of Effective Region
~1.5 kb
Overview of Gene Research
ARID5B, a member of the AT-rich interactive domain (ARID) protein family, has been implicated in multiple biological processes. It may play a role in chromatin remodeling as part of the SWI/SNF chromatin remodelling complex [3]. It is also involved in regulating gene expression, with potential implications in normal and malignant hematopoiesis, B-cell development, and function [1,2].
In mouse models, Arid5b overexpression led to a reduction in the proportion of circulating B cells, immature and mature B-cell fractions in the peripheral blood and bone marrow, and follicular B cells in the spleen. There were also defects in B-cell activation and increased mitochondrial oxygen consumption rate in B cells [2]. In addition, deletion of Arid5b in mice resulted in a decrease in the proportion of immature B cells but an increase in large and small Pre-B cells, along with enhanced fatty acid uptake and oxidation in Pre-B cells. Decreased ARID5B expression in humans is a risk factor for B-cell leukemia [4].
Overall, ARID5B is crucial for B-cell development and function, regulating fatty acid metabolism and proliferation at the Pre-B cell stage. Mouse models, especially those with Arid5b overexpression or deletion, have provided valuable insights into its role in B-cell-related processes and the risk of B-cell leukemia [2,4].
References：
1. Wang, Peiqi, Deng, Yun, Yan, Xinyu, Xu, Heng, Lu, Xiaoxi. 2020. The Role of ARID5B in Acute Lymphoblastic Leukemia and Beyond. In Frontiers in genetics, 11, 598. doi:10.3389/fgene.2020.00598. https://pubmed.ncbi.nlm.nih.gov/32595701/
2. Goodings, Charnise, Zhao, Xujie, McKinney-Freeman, Shannon, Zhang, Hui, Yang, Jun J. 2023. ARID5B influences B-cell development and function in mouse. In Haematologica, 108, 502-512. doi:10.3324/haematol.2022.281157. https://pubmed.ncbi.nlm.nih.gov/35924577/
3. Kandoth, Cyriac, Schultz, Nikolaus, Cherniack, Andrew D, Mardis, Elaine R, Levine, Douglas A. . Integrated genomic characterization of endometrial carcinoma. In Nature, 497, 67-73. doi:10.1038/nature12113. https://pubmed.ncbi.nlm.nih.gov/23636398/
4. Chalise, Jaya Prakash, Ehsani, Ali, Lemecha, Mengistu, Larson, Garrett P, Itakura, Keiichi. 2023. ARID5B regulates fatty acid metabolism and proliferation at the Pre-B cell stage during B cell development. In Frontiers in immunology, 14, 1170475. doi:10.3389/fimmu.2023.1170475. https://pubmed.ncbi.nlm.nih.gov/37483604/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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