C57BL/6JCya-Art1em1/Cya
Common Name:
Art1-KO
Product ID:
S-KO-17302
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Art1-KO
Strain ID
KOCMP-11870-Art1-B6J-VB
Gene Name
Product ID
S-KO-17302
Gene Alias
ADPRT; ARTC1; Yac-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Art1em1/Cya mice (Catalog S-KO-17302) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033300
NCBI RefSeq
NM_009710
Target Region
Exon 2~3
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Art1, also known as Arginine-specific mono-ADP-ribosyltransferase-1 or Aluminum Resistance Transcription Factor 1, has diverse functions. In mammals, it positively regulates inflammatory cytokines and is involved in the IL-6 signaling pathway [1]. In plants, especially rice, it contributes to aluminum resistance by modifying cell wall properties [2].
In colorectal cancer cells, ART1 knockdown reduces IL-6-induced cell proliferation, with decreased viability, colony formation, DNA synthesis, and down-regulation of proteins like gp130, c-Myc, cyclin D1, Bcl-xL, and the p-STAT3/STAT3 ratio. In vivo, mice transplanted with ART1-sh CT26 cells and high IL-6 levels had smaller tumors [1]. In non-small cell lung cancer, tumor cell-expressed ART1 mediates immune resistance by causing ADP-ribosylation and cell death of P2X7R+ CD8 T cells, and its inhibition slows tumor growth in a CD8 T cell-dependent manner [3]. In rice, art1 mutant shows reduced aluminum resistance, and exogenous putrescine can restore it. ART1 directly inhibits the expression of OsMYB30 to enhance aluminum resistance [2]. Overexpression of an ART1-interacting gene OsNAC016 in rice improves aluminum tolerance by regulating certain cell-wall-related or ART-regulated genes [4].
In conclusion, Art1 plays crucial roles in inflammation-related cancer cell proliferation and immune resistance in mammals, as well as aluminum resistance in plants. Studies using gene knockdown or knockout models in cancer and plant systems have revealed these functions, providing potential therapeutic targets for human cancers and insights into plant stress resistance mechanisms.
References:
1. Lin, Ting, Zhang, Shuxian, Tang, Yi, Xie, Hailun, Wang, Yalan. 2024. ART1 knockdown decreases the IL-6-induced proliferation of colorectal cancer cells. In BMC cancer, 24, 354. doi:10.1186/s12885-024-12120-0. https://pubmed.ncbi.nlm.nih.gov/38504172/
2. Gao, Li Jun, Liu, Xiang Pei, Gao, Ke Ke, Harberd, Nicholas P, Zheng, Shao Jian. 2023. ART1 and putrescine contribute to rice aluminum resistance via OsMYB30 in cell wall modification. In Journal of integrative plant biology, 65, 934-949. doi:10.1111/jipb.13429. https://pubmed.ncbi.nlm.nih.gov/36515424/
3. Wennerberg, Erik, Mukherjee, Sumit, Spada, Sheila, McGraw, Timothy E, Stiles, Brendon M. 2022. Expression of the mono-ADP-ribosyltransferase ART1 by tumor cells mediates immune resistance in non-small cell lung cancer. In Science translational medicine, 14, eabe8195. doi:10.1126/scitranslmed.abe8195. https://pubmed.ncbi.nlm.nih.gov/35294260/
4. Liu, Fuhang, Ma, Dan, Yu, Jinyu, Peng, Liyun, Xia, Jixing. 2023. Overexpression of an ART1-Interacting Gene OsNAC016 Improves Al Tolerance in Rice. In International journal of molecular sciences, 24, . doi:10.3390/ijms242317036. https://pubmed.ncbi.nlm.nih.gov/38069359/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen