C57BL/6JCya-Crotem1/Cya
Common Name:
Crot-KO
Product ID:
S-KO-17309
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Crot-KO
Strain ID
KOCMP-74114-Crot-B6J-VB
Gene Name
Product ID
S-KO-17309
Gene Alias
1200003H03Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crotem1/Cya mice (Catalog S-KO-17309) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003720
NCBI RefSeq
NM_023733
Target Region
Exon 4
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Crot, short for Carnitine O-octanoyltransferase, is a well-established peroxisomal enzyme. It is involved in liver fatty acid oxidation, and recent research has explored its role in promoting vascular calcification. It also participates in lipid metabolism-related pathways, and its function is of great biological importance [1,2]. Genetic models, such as knockout mouse models, have been crucial for studying its functions.
In Crot-deficient (Crot-/-) mice, metabolomic analysis showed that omega-3 fatty acids like EPA, DPA, and DHA were increased in the liver, and EPA was higher in the plasma. Anti-inflammatory dicarboxylic acids were also elevated in the plasma of Crot-deficient mice, suggesting a potential anti-calcification mechanism of Crot suppression [1]. In addition, Nuclease technology-mediated Crot deficiency in LDL receptor-deficient mice reduced aortic and carotid artery calcification without affecting bone density or lipid levels in the liver and plasma, indicating Crot's role in vascular calcification via promoting fatty acid metabolism and mitochondrial dysfunction [2].
In conclusion, Crot is essential in liver fatty acid oxidation and lipid metabolism. Studies using Crot knockout mouse models have revealed its significant role in vascular calcification, suggesting that Crot inhibition may be a potential antifibrocalcific therapy. These models have provided valuable insights into the function of Crot in specific disease-related biological processes [1,2].
References:
1. Okui, Takehito, Kuraoka, Shiori, Iwashita, Masaya, Singh, Sasha A, Aikawa, Elena. 2024. Carnitine O-octanoyltransferase (CROT) deficiency in mice leads to an increase of omega-3 fatty acids. In Frontiers in molecular biosciences, 11, 1374316. doi:10.3389/fmolb.2024.1374316. https://pubmed.ncbi.nlm.nih.gov/39076376/
2. Okui, Takehito, Iwashita, Masaya, Rogers, Maximillian A, Singh, Sasha A, Aikawa, Elena. 2020. CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing Factor in Vascular Calcification via Promoting Fatty Acid Metabolism and Mitochondrial Dysfunction. In Arteriosclerosis, thrombosis, and vascular biology, 41, 755-768. doi:10.1161/ATVBAHA.120.315007. https://pubmed.ncbi.nlm.nih.gov/33356393/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen