C57BL/6JCya-Dhx32em1/Cya
Common Name:
Dhx32-KO
Product ID:
S-KO-17325
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dhx32-KO
Strain ID
KOCMP-101437-Dhx32-B6J-VB
Gene Name
Product ID
S-KO-17325
Gene Alias
4732469F02Rik; Ddx32; muDDX32
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dhx32em1/Cya mice (Catalog S-KO-17325) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033290
NCBI RefSeq
NM_133941
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
DHX32, also known as DEAH-box polypeptide 32, is an RNA helicase. It is involved in numerous RNA-associated biological processes such as ribosome biosynthesis, transcription, mRNA splicing and translation [5].
In cancer research, in liver cancer, its knockdown enhanced the proliferative potential of liver cancer cells, with upregulated phosphorylated levels of ERK, Akt and increased CDK6 level, indicating it may have a proliferation-suppressing property [1]. In breast cancer, increased DHX32 expression was associated with poor prognosis and was an independent prognostic factor for decreased overall and disease-free survival [2]. In colorectal cancer, DHX32 promoted angiogenesis by upregulating VEGFA through interacting with and stabilizing β-catenin, and overexpression of DHX32 contributed to cell growth, metastasis and decreased chemotherapy susceptibility [3,4]. In hepatocellular carcinoma, high DHX32 expression was associated with reduced overall survival, and its ectopic expression induced EMT, promoted cell mobility, proliferation and tumour growth, while silencing reversed these effects via the β-catenin pathway [6].
In conclusion, DHX32 is an important RNA helicase with key functions in RNA-related biological processes. Research on DHX32, especially through loss-of-function experiments in cancer cells, reveals its significant role in cancer progression, making it a potential biomarker and therapeutic target for various cancers such as liver, breast, colorectal and hepatocellular carcinoma.
References:
1. Cai, Min-Jing, Zhu, Jian-Hui, He, Jian-Quan, Zhang, Zhong-Ying, Liang, Xian-Ming. . Silencing of DHX32 increases the proliferation of liver cancer cells. In Translational cancer research, 9, 1833-1842. doi:10.21037/tcr.2020.02.35. https://pubmed.ncbi.nlm.nih.gov/35117530/
2. Wang, Meng, Zhang, Guojun, Wang, Yajie, Fang, Fang, Kang, Xixiong. 2016. DHX32 expression is an indicator of poor breast cancer prognosis. In Oncology letters, 13, 942-948. doi:10.3892/ol.2016.5503. https://pubmed.ncbi.nlm.nih.gov/28356982/
3. Lin, Huayue, Fang, Zanxi, Su, Yuanhui, Wang, Fen, Zhang, Zhong-Ying. 2017. DHX32 Promotes Angiogenesis in Colorectal Cancer Through Augmenting β-catenin Signaling to Induce Expression of VEGFA. In EBioMedicine, 18, 62-72. doi:10.1016/j.ebiom.2017.03.012. https://pubmed.ncbi.nlm.nih.gov/28330603/
4. Lin, Huayue, Liu, Wenjuan, Fang, Zanxi, Wang, Fen, Zhang, Zhong-Ying. 2015. Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer. In Scientific reports, 5, 9247. doi:10.1038/srep09247. https://pubmed.ncbi.nlm.nih.gov/25782664/
5. Wei, Qingchun, Geng, Jinting, Chen, Yongquan, Wang, Fen, Zhang, Zhongying. 2021. Structure and function of DEAH-box helicase 32 and its role in cancer. In Oncology letters, 21, 382. doi:10.3892/ol.2021.12643. https://pubmed.ncbi.nlm.nih.gov/33777205/
6. Hu, Xiaoyun, Yuan, Guosheng, Li, Qi, Cheng, Xiao, Chen, Jinzhang. . DEAH-box polypeptide 32 promotes hepatocellular carcinoma progression via activating the β-catenin pathway. In Annals of medicine, 53, 437-447. doi:10.1080/07853890.2021.1898674. https://pubmed.ncbi.nlm.nih.gov/33729094/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen