C57BL/6JCya-Mcrs1em1/Cya
Common Name:
Mcrs1-KO
Product ID:
S-KO-17356
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mcrs1-KO
Strain ID
KOCMP-51812-Mcrs1-B6J-VA
Gene Name
Product ID
S-KO-17356
Gene Alias
ICP22BP; MSP58; P78
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcrs1em1/Cya mice (Catalog S-KO-17356) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041190
NCBI RefSeq
NM_016766
Target Region
Exon 7~8
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Mcrs1, also known as Microspherule protein 1 or MSP58, is a multifunctional protein involved in diverse cellular processes. It is a component of the nonspecific lethal (NSL) complex and the chromatin remodeling INO80 complex, participating in transcriptional regulation during mitosis. Mcrs1 is crucial for normal development, cell cycle regulation, and has implications in diseases like cancer [2,3,4,5,6]. Genetic models, such as KO/CKO mouse models, have been instrumental in studying its functions.
In mouse oocytes, Mcrs1 depletion leads to G2/M transition delay and abnormal spindle assembly, indicating its role in cell cycle regulation and spindle organization during meiosis [2]. In early mouse embryogenesis, Mcrs1 mutant embryos show normal blastocyst morphology but fail to implant, with compromised epiblast lineage development [4]. In craniofacial development, Mcrs1 knockdown affects the expression of genes related to otic vesicles and branchial arches, leading to cartilage formation defects [3]. In solid tumors, MCRS1 can augment MHC-I expression, sensitizing cancer cells to T cell immunity and α-PD-1 therapy [1]. In gastric cancer, high MCRS1 expression is associated with increased tumor activity and poor patient survival [5].
In summary, Mcrs1 plays essential roles in cell cycle regulation, embryonic development, and craniofacial cartilage formation. In disease areas, its function in modulating tumor-immune interactions in solid tumors and influencing tumor activity in gastric cancer is significant. Studies using KO/CKO mouse models have greatly enhanced our understanding of Mcrs1's biological functions and its implications in disease.
References:
1. Li, Xue, Yi, Han, Jin, Zheyu, Liu, Yanfang, Yu, Xiaofei. 2024. MCRS1 sensitizes T cell-dependent immunotherapy by augmenting MHC-I expression in solid tumors. In The Journal of experimental medicine, 221, . doi:10.1084/jem.20240959. https://pubmed.ncbi.nlm.nih.gov/39545935/
2. Ju, Jia-Qian, Pan, Zhen-Nan, Zhang, Kun-Huan, Liu, Jing-Cai, Sun, Shao-Chen. 2023. Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis. In EMBO reports, 24, e56273. doi:10.15252/embr.202256273. https://pubmed.ncbi.nlm.nih.gov/36951681/
3. Keer, Stephanie, Cousin, Helene, Jourdeuil, Karyn, Alfandari, Dominique, Moody, Sally A. 2022. Mcrs1 is required for branchial arch and cranial cartilage development. In Developmental biology, 489, 62-75. doi:10.1016/j.ydbio.2022.06.002. https://pubmed.ncbi.nlm.nih.gov/35697116/
4. Cui, Wei, Cheong, Agnes, Wang, Yongsheng, Tremblay, Kimberly D, Mager, Jesse. . MCRS1 is essential for epiblast development during early mouse embryogenesis. In Reproduction (Cambridge, England), 159, 1-13. doi:10.1530/REP-19-0334. https://pubmed.ncbi.nlm.nih.gov/31671403/
5. Wang, Liang-Han, Chang, Chih-Chun, Cheng, Chiao-Yin, Sun, Jen-Tang, Chen, Yen-Lin. 2022. MCRS1 Expression Regulates Tumor Activity and Affects Survival Probability of Patients with Gastric Cancer. In Diagnostics (Basel, Switzerland), 12, . doi:10.3390/diagnostics12061502. https://pubmed.ncbi.nlm.nih.gov/35741311/
6. Huang, Ching-Jung, Lyu, Xiaoai, Kang, Jungseog. 2022. The molecular characteristics and functional roles of microspherule protein 1 (MCRS1) in gene expression, cell proliferation, and organismic development. In Cell cycle (Georgetown, Tex.), 22, 619-632. doi:10.1080/15384101.2022.2145816. https://pubmed.ncbi.nlm.nih.gov/36384428/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen