C57BL/6JCya-Senp8em1/Cya
Common Name:
Senp8-KO
Product ID:
S-KO-17362
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Senp8-KO
Strain ID
KOCMP-71599-Senp8-B6J-VA
Gene Name
Product ID
S-KO-17362
Gene Alias
9130010J17Rik; Den1; Nedp1; Prsc2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Senp8em1/Cya mice (Catalog S-KO-17362) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000216329
NCBI RefSeq
NM_001172071
Target Region
Exon 3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Senp8, also known as human deneddylase-1, is a SUMO peptidase family member that specifically acts as a deneddylating enzyme. Neddylation is a cellular process where NEDD8 is conjugated to target proteins, and Senp8 counteracts this by removing NEDD8 from over-neddylated proteins. It is involved in multiple pathways such as actin dynamics, Wnt/β-catenin signaling, and autophagic processes, and plays a crucial role in maintaining proper neddylation levels for cullin-RING ubiquitin ligase (CRL)-dependent proteostasis [5,6].
In neuronal development, Senp8 negatively regulates neurite outgrowth, which subsequently impairs excitatory synapse maturation. Its expression levels are developmentally regulated, peaking around the first postnatal week and then diminishing in mature brain and neurons [1]. In hepatitis B virus (HBV) propagation, overexpression of Senp8, which cleaves conjugated NEDD8, suppresses HBV propagation, indicating that neddylation plays an important role in the late stages of HBV life cycles [2]. In colon cancer, Senp8-mediated SHP2 deneddylation via the CD47/SIRPα axis mediates tumor immunosuppression [3]. In acute lymphoblastic leukemia (ALL), SENP8 is highly expressed in relapsed specimens, and overexpressing SENP8 reduces the chemosensitivity of ALL cells to etoposide, while knockdown of SENP8 can sensitize cells to the drug [4].
In conclusion, Senp8 is a key deneddylating enzyme with essential functions in neuronal development, viral propagation, tumor immunosuppression, and drug resistance in leukemia. Studies on Senp8, especially through loss-of-function experiments in relevant models, have provided insights into its role in these biological processes and disease conditions, potentially offering new therapeutic targets for neurodevelopmental disorders, HBV-related diseases, and certain cancers.
References:
1. Song, Jae-Man, Kang, Minji, Lee, Seungha, Lee, Sanghyeon, Suh, Young Ho. 2023. Deneddylating enzyme SENP8 regulates neuronal development. In Journal of neurochemistry, 165, 348-361. doi:10.1111/jnc.15797. https://pubmed.ncbi.nlm.nih.gov/36847487/
2. Chen, David Virya, Suzuki, Tatsuya, Itoh, Yumi, Matsuura, Yoshiharu, Okamoto, Toru. 2021. Deneddylation by SENP8 restricts hepatitis B virus propagation. In Microbiology and immunology, 65, 125-135. doi:10.1111/1348-0421.12874. https://pubmed.ncbi.nlm.nih.gov/33433029/
3. Li, Yiqing, Zhou, Hui, Liu, Pan, Zhang, Xue, Ke, Yuehai. 2023. SHP2 deneddylation mediates tumor immunosuppression in colon cancer via the CD47/SIRPα axis. In The Journal of clinical investigation, 133, . doi:10.1172/JCI162870. https://pubmed.ncbi.nlm.nih.gov/36626230/
4. Sun, Shuzhang, Cheng, Yixuan, Hou, Wanxin, Li, Hegen, Xiao, Ning. 2024. Etoposide-induced SENP8 confers a feed-back drug resistance on acute lymphoblastic leukemia cells. In Biochemistry and biophysics reports, 37, 101650. doi:10.1016/j.bbrep.2024.101650. https://pubmed.ncbi.nlm.nih.gov/38314144/
5. Chen, Jonathan J, Schmucker, Lyndsey N, Visco, Donald P. 2019. Identifying de-NEDDylation inhibitors: Virtual high-throughput screens targeting SENP8. In Chemical biology & drug design, 93, 590-604. doi:10.1111/cbdd.13457. https://pubmed.ncbi.nlm.nih.gov/30560590/
6. Coleman, Kate E, Békés, Miklós, Chapman, Jessica R, Ueberheide, Beatrix M, Huang, Tony T. 2017. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. In eLife, 6, . doi:10.7554/eLife.24325. https://pubmed.ncbi.nlm.nih.gov/28475037/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen