C57BL/6JCya-Nctc1em1/Cya
Common Name:
Nctc1-KO
Product ID:
S-KO-17368
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nctc1-KO
Strain ID
KOCMP-330677-Nctc1-B6J-VA
Gene Name
Product ID
S-KO-17368
Gene Alias
9530074L01; Rhit1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nctc1em1/Cya mice (Catalog S-KO-17368) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000132167
NCBI RefSeq
NR_002452
Target Region
Exon 1~3
Size of Effective Region
~18.8 kb
Detailed Document
Overview of Gene Research
Nctc1 is a long non-coding RNA gene. It is located within the Igf2-H19-Nctc1 locus, and genes in this locus are important for studying monoallelic expression mechanisms and the role of cis-regulatory elements in chromatin organization and gene expression [2,3].
H19 lncRNA knockdown leads to increased DNMT3B-mediated methylation of Nctc1. This suggests that H19 lncRNA can regulate the methylation of Nctc1, and genome-wide methylation changes associated with H19-mediated SAHH modulation are consistent with an impact on Nctc1 methylation [1]. Nctc1 is co-regulated with Igf2 and H19, and all three genes can physically interact with a shared muscle enhancer and with each other via enhancer interactions. Promoter-promoter cross-talk through this shared enhancer explains the paternally biased expression of Nctc1 [3]. Also, transcription of Nctc1 in cis is essential for the function of the Igf2/H19 muscle enhancer, indicating it is part of an active transcriptional complex [4].
In conclusion, Nctc1 is a key component in the regulation of gene expression at the Igf2-H19 locus. Its regulation by H19 lncRNA methylation and its role in enhancer-mediated promoter-promoter cross-talk contribute to understanding the epigenetic and transcriptional regulation mechanisms. These findings may be relevant for understanding developmental processes and potentially related diseases associated with the abnormal regulation of this locus [1,3,4].
References：
1. Zhou, Jichun, Yang, Lihua, Zhong, Tianyu, Taylor, Hugh S, Huang, Yingqun. 2015. H19 lncRNA alters DNA methylation genome wide by regulating S-adenosylhomocysteine hydrolase. In Nature communications, 6, 10221. doi:10.1038/ncomms10221. https://pubmed.ncbi.nlm.nih.gov/26687445/
2. Kim, Jae-Young, Youn, Hwa Young, Pfeifer, Karl, Eun, Bokkee. 2019. Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells. In Animal cells and systems, 23, 288-293. doi:10.1080/19768354.2019.1612780. https://pubmed.ncbi.nlm.nih.gov/31489250/
3. Eun, Bokkee, Sampley, Megan L, Good, Austin L, Gebert, Claudia M, Pfeifer, Karl. 2012. Promoter cross-talk via a shared enhancer explains paternally biased expression of Nctc1 at the Igf2/H19/Nctc1 imprinted locus. In Nucleic acids research, 41, 817-26. doi:10.1093/nar/gks1182. https://pubmed.ncbi.nlm.nih.gov/23221643/
4. Eun, Bokkee, Sampley, Megan L, Van Winkle, Matthew T, Kachman, Marika M, Pfeifer, Karl. 2013. The Igf2/H19 muscle enhancer is an active transcriptional complex. In Nucleic acids research, 41, 8126-34. doi:10.1093/nar/gkt597. https://pubmed.ncbi.nlm.nih.gov/23842673/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen