C57BL/6JCya-Ap2b1em1/Cya
Common Name:
Ap2b1-KO
Product ID:
S-KO-17401
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ap2b1-KO
Strain ID
KOCMP-71770-Ap2b1-B6J-VB
Gene Name
Product ID
S-KO-17401
Gene Alias
1300012O03Rik; AP105B
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ap2b1em1/Cya mice (Catalog S-KO-17401) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000018875
NCBI RefSeq
NM_001035854
Target Region
Exon 4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ap2b1, also known as adaptor-related protein complex 2 subunit beta 1, is involved in the endocytosis process, a crucial cellular mechanism for internalizing extracellular materials. It is associated with pathways such as clathrin-mediated endocytosis and caveola-mediated endocytosis, which are vital for maintaining cellular homeostasis, nutrient uptake, and signal transduction [4,5].
In the context of various diseases, in high-altitude cerebral edema, up-regulated nuclear respiratory factor 1 (NRF1) was found to up-regulate Ap2b1 in activated microglia under hypoxia, enhancing phagocytic function and contributing to the development of the disease [1]. In a male mouse model of noise-induced sensorineural hearing loss, miR-145b negatively regulated Ap2b1, and depletion of miR-145b alleviated auditory threshold shifts and outer hair cell loss by upregulating Ap2b1 expression [2]. In Parkinson's disease and related disorders, lower levels of AP2B1 were observed in the cerebrospinal fluid (CSF) of patients compared to healthy controls, suggesting its potential as a biomarker for synaptic dysfunction [3]. Regarding viral infections, LINC08148 knockout downregulated the transcription levels of Ap2b1 among other endocytosis-related genes, reducing the replication of Zika virus [4]. Also, the G protein-coupled receptor FFAR2 was shown to interact with β-arrestin1 which in turn interacted with AP2B1, and knockdown of AP2B1 impaired influenza A virus replication [5]. In Alzheimer's disease, differences in the CSF concentration of AP2B1 were observed between patients and healthy controls, indicating its possible role in the disease's pathogenesis [6,8]. In major depressive disorder, AP2B1 was identified as a hub gene in a protein-protein interaction network, suggesting it could be a potential therapeutic target [7]. In the study of hUC-MSC extracellular vesicles, AP2A1 and AP2B1 may play a role in treating Alzheimer's disease by regulating the synaptic vesicle cycle signalling pathway [9].
In conclusion, Ap2b1 is essential for endocytosis-related cellular functions. Studies using various models, though not always gene-knockout models, have revealed its significance in multiple disease areas including high-altitude cerebral edema, sensorineural hearing loss, neurodegenerative diseases, viral infections, and major depressive disorder. These findings provide insights into potential therapeutic strategies and biomarker development related to these diseases.
References:
1. Wang, Xueting, Chen, Guijuan, Wan, Baolan, Lu, Yapeng, Zhu, Li. . NRF1-mediated microglial activation triggers high-altitude cerebral edema. In Journal of molecular cell biology, 14, . doi:10.1093/jmcb/mjac036. https://pubmed.ncbi.nlm.nih.gov/35704676/
2. Gu, Xiang, Jiang, Mengxian, Chen, Wei. 2025. miR-145b/AP2B1 Axis Contributes to Noise-induced Sensorineural Hearing Loss In a Male Mouse Model. In Cell biochemistry and biophysics, , . doi:10.1007/s12013-024-01665-3. https://pubmed.ncbi.nlm.nih.gov/39813009/
3. Nilsson, Johanna, Constantinescu, Julius, Nellgård, Bengt, Bäckström, David, Brinkmalm, Ann. 2022. Cerebrospinal Fluid Biomarkers of Synaptic Dysfunction are Altered in Parkinson's Disease and Related Disorders. In Movement disorders : official journal of the Movement Disorder Society, 38, 267-277. doi:10.1002/mds.29287. https://pubmed.ncbi.nlm.nih.gov/36504237/
4. Huo, Zhiting, Zhu, Xuanfeng, Peng, Qinyu, Liu, Chao, Zhang, Ping. 2024. LINC08148 promotes the caveola-mediated endocytosis of Zika virus through upregulating transcription of Src. In Journal of virology, 98, e0170523. doi:10.1128/jvi.01705-23. https://pubmed.ncbi.nlm.nih.gov/38742902/
5. Wang, Guangwen, Jiang, Li, Wang, Jinliang, Chen, Hualan, Li, Chengjun. 2020. The G Protein-Coupled Receptor FFAR2 Promotes Internalization during Influenza A Virus Entry. In Journal of virology, 94, . doi:10.1128/JVI.01707-19. https://pubmed.ncbi.nlm.nih.gov/31694949/
6. Krance, Saffire H, Wu, Che-Yuan, Chan, Alison C Y, Lanctôt, Krista L, Swardfager, Walter. . Endosomal-Lysosomal and Autophagy Pathway in Alzheimer's Disease: A Systematic Review and Meta-Analysis. In Journal of Alzheimer's disease : JAD, 88, 1279-1292. doi:10.3233/JAD-220360. https://pubmed.ncbi.nlm.nih.gov/35754279/
7. Feng, Jianfei, Zhou, Qing, Gao, Wenquan, Wu, Yanying, Mu, Ruibin. 2019. Seeking for potential pathogenic genes of major depressive disorder in the Gene Expression Omnibus database. In Asia-Pacific psychiatry : official journal of the Pacific Rim College of Psychiatrists, 12, e12379. doi:10.1111/appy.12379. https://pubmed.ncbi.nlm.nih.gov/31889427/
8. Sjödin, Simon, Brinkmalm, Gunnar, Öhrfelt, Annika, Zetterberg, Henrik, Brinkmalm, Ann. 2019. Endo-lysosomal proteins and ubiquitin CSF concentrations in Alzheimer's and Parkinson's disease. In Alzheimer's research & therapy, 11, 82. doi:10.1186/s13195-019-0533-9. https://pubmed.ncbi.nlm.nih.gov/31521194/
9. Li, Shuang, Zhang, Jiayi, Liu, Xinxing, Guo, Chunyan, Liu, Xifu. 2024. Proteomic characterization of hUC-MSC extracellular vesicles and evaluation of its therapeutic potential to treat Alzheimer's disease. In Scientific reports, 14, 5959. doi:10.1038/s41598-024-56549-6. https://pubmed.ncbi.nlm.nih.gov/38472335/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen