C57BL/6JCya-Pxdnem1/Cya
Common Name:
Pxdn-KO
Product ID:
S-KO-17412
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pxdn-KO
Strain ID
KOCMP-69675-Pxdn-B6J-VB
Gene Name
Product ID
S-KO-17412
Gene Alias
2310075M15Rik; E330004E07; VPO1; mKIAA0230
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pxdnem1/Cya mice (Catalog S-KO-17412) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000122328
NCBI RefSeq
NM_181395
Target Region
Exon 7~9
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Peroxidasin (PXDN), also known as Vascular Peroxidase-1 (VPO1), is a heme-containing peroxidase. It catalyzes the oxidation of various substrates using hydrogen peroxide (H2O2), playing diverse roles in physiology and pathology. It is involved in innate immunity, cardiovascular physiology and diseases, extracellular matrix formation, and has been associated with pathways like autophagy, oxidative stress, and inflammation [1]. Genetic models, such as gene knockout mice, are valuable for studying its functions.
In mice, biallelic deletion of Pxdn using the Nuclease technology system led to anophthalmia and severe eye malformation, indicating PXDN's essential role in eye development. Homozygous mice showed extreme eye-development defects, while heterozygous mice had normal eye structure and function, suggesting a single allele of the Pxdn gene suffices for normal eye-structure formation and visual function [3]. In addition, in an elastase-induced mouse abdominal aortic aneurysm (AAA) model, alpha-ketoglutarate (AKG) treatment suppressed the development of AAA by inhibiting the PXDN/HOCL/ERK signaling pathways [2].
In conclusion, PXDN is crucial for multiple biological processes. Its role in eye development is clearly demonstrated through Pxdn knockout mouse models. In disease areas, the study of PXDN in mouse models provides insights into conditions like abdominal aortic aneurysm, highlighting its potential as a therapeutic target.
References:
1. Cheng, Guangjie, Shi, Ruizheng. 2022. Mammalian peroxidasin (PXDN): From physiology to pathology. In Free radical biology & medicine, 182, 100-107. doi:10.1016/j.freeradbiomed.2022.02.026. https://pubmed.ncbi.nlm.nih.gov/35219848/
2. Liu, Junjun, Liu, Mingyuan, Feng, Jiaxuan, Niu, Haitao, Guo, Mingjin. 2022. Alpha-ketoglutarate ameliorates abdominal aortic aneurysm via inhibiting PXDN/HOCL/ERK signaling pathways. In Journal of translational medicine, 20, 461. doi:10.1186/s12967-022-03659-2. https://pubmed.ncbi.nlm.nih.gov/36209172/
3. Kim, Hyun-Kyung, Ham, Kyung A, Lee, Seung-Woo, Kim, In-Beom, Joe, Young Ae. 2019. Biallelic Deletion of Pxdn in Mice Leads to Anophthalmia and Severe Eye Malformation. In International journal of molecular sciences, 20, . doi:10.3390/ijms20246144. https://pubmed.ncbi.nlm.nih.gov/31817535/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen