C57BL/6JCya-Stab2em1/Cya
Common Name:
Stab2-KO
Product ID:
S-KO-17430
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Stab2-KO
Strain ID
KOCMP-192188-Stab2-B6J-VB
Gene Name
Product ID
S-KO-17430
Gene Alias
FEEL-2; FELL; MFEEL-2; STAB-2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Stab2em1/Cya mice (Catalog S-KO-17430) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035288
NCBI RefSeq
NM_138673
Target Region
Exon 4
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Stab2, also known as Stabilin-2, is a scavenger receptor preferentially expressed by liver sinusoidal endothelial cells. It mediates the clearance of various circulating plasma molecules, thus playing a crucial role in maintaining distant organ homeostasis. It is involved in the clearance of glycosaminoglycans (GAGs), apoptotic cells, and bacteria, and is responsible for the specific binding and internalization of 22 discrete molecules, macromolecular complexes, and cell types [5].
In gene-knockout (KO) mouse models, studies have shown significant impacts on disease-related processes. ApoE-Stab2-KO mice had reduced spontaneous and Western diet-associated atherogenesis, indicating that Stab2 may contribute to atherogenesis [1]. Similarly, Stab2-/-ApoE-/-males on a Western diet developed approximately 30% smaller atherosclerotic plaques, suggesting that reduction of Stab2 is protective against atherosclerotic plaque development [4]. Whole-exome sequencing in humans identified rare variants in STAB2 associated with venous thromboembolic disease, with VTE-associated variants having reduced surface expression [3]. In zebrafish, gene-edited zebrafish lacking Stabilin-2 showed abrogated interaction of fibrillar supramolecular nanoparticles with the caudal vein, demonstrating its role in nanoparticle clearance [2].
In conclusion, Stab2 is essential for the clearance of multiple molecules and plays a significant role in maintaining physiological homeostasis. KO mouse models and other functional studies have revealed its importance in diseases such as atherosclerosis and venous thromboembolic disease. Understanding Stab2's functions through these models provides insights into disease mechanisms and potential therapeutic targets for these diseases.
References:
1. Manta, Calin-Petru, Leibing, Thomas, Friedrich, Mirco, Goerdt, Sergij, Géraud, Cyrill. 2022. Targeting of Scavenger Receptors Stabilin-1 and Stabilin-2 Ameliorates Atherosclerosis by a Plasma Proteome Switch Mediating Monocyte/Macrophage Suppression. In Circulation, 146, 1783-1799. doi:10.1161/CIRCULATIONAHA.121.058615. https://pubmed.ncbi.nlm.nih.gov/36325910/
2. Saez Talens, Victorio, Arias-Alpizar, Gabriela, Makurat, D M M, Kros, Alexander, Kieltyka, Roxanne E. 2020. Stab2-Mediated Clearance of Supramolecular Polymer Nanoparticles in Zebrafish Embryos. In Biomacromolecules, 21, 1060-1068. doi:10.1021/acs.biomac.9b01318. https://pubmed.ncbi.nlm.nih.gov/32083854/
3. Desch, Karl C, Ozel, Ayse B, Halvorsen, Matt, Goldstein, David, Ginsburg, David. . Whole-exome sequencing identifies rare variants in STAB2 associated with venous thromboembolic disease. In Blood, 136, 533-541. doi:10.1182/blood.2019004161. https://pubmed.ncbi.nlm.nih.gov/32457982/
4. Kayashima, Yukako, Clanton, Connor A, Lewis, Amanda M, Maeda-Smithies, Nobuyo, Harris, Edward N. 2022. Reduction of Stabilin-2 Contributes to a Protection Against Atherosclerosis. In Frontiers in cardiovascular medicine, 9, 818662. doi:10.3389/fcvm.2022.818662. https://pubmed.ncbi.nlm.nih.gov/35360009/
5. Weigel, Paul H. 2020. Systemic Glycosaminoglycan Clearance by HARE/Stabilin-2 Activates Intracellular Signaling. In Cells, 9, . doi:10.3390/cells9112366. https://pubmed.ncbi.nlm.nih.gov/33126404/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen