C57BL/6JCya-Mrap2em1/Cya
Common Name:
Mrap2-KO
Product ID:
S-KO-17439
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mrap2-KO
Strain ID
KOCMP-244958-Mrap2-B6J-VB
Gene Name
Product ID
S-KO-17439
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mrap2em1/Cya mice (Catalog S-KO-17439) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113149
NCBI RefSeq
XM_006511179
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
MRAP2, the melanocortin receptor-associated protein 2, is crucial for regulating energy homeostasis. It is involved in multiple pathways related to appetite control and body weight regulation, interacting with several G-protein-coupled receptors (GPCRs) such as the melanocortin-4 receptor (MC4R), ghrelin receptor (GHSR1a), and prokineticin receptors (PKR1 and PKR2) [1,2,3,4]. Genetic models, like MRAP2 knockout mice, have been valuable in understanding its functions.
In MRAP2 knockout mice, the orexigenic effect of ghrelin is lost, and fasting fails to activate agouti-related protein neurons, indicating MRAP2's role in hunger sensing and energy homeostasis regulation through modulation of ghrelin-GHSR1a signaling [3]. Also, MRAP2 knockout mice display an obese phenotype, similar to MC3R and MC4R knockout mice, suggesting its importance in controlling appetite and energy balance [5]. In addition, human mutations in MRAP2 are associated with monogenic hyperphagic obesity with hyperglycaemia and hypertension [5].
In conclusion, MRAP2 is a key regulator in energy homeostasis, playing a significant role in appetite control, hunger sensing, and body weight regulation. The study of MRAP2 knockout mouse models has provided insights into its functions in the context of obesity and related metabolic disorders, highlighting its potential as a therapeutic target for treating obesity and associated metabolic diseases.
References：
1. Bernard, Adelaide, Ojeda Naharros, Irene, Yue, Xinyu, Reiter, Jeremy F, Vaisse, Christian. 2023. MRAP2 regulates energy homeostasis by promoting primary cilia localization of MC4R. In JCI insight, 8, . doi:10.1172/jci.insight.155900. https://pubmed.ncbi.nlm.nih.gov/36692018/
2. Rouault, Alix A J, Buscaglia, Paul, Sebag, Julien A. 2022. MRAP2 inhibits β-arrestin recruitment to the ghrelin receptor by preventing GHSR1a phosphorylation. In The Journal of biological chemistry, 298, 102057. doi:10.1016/j.jbc.2022.102057. https://pubmed.ncbi.nlm.nih.gov/35605660/
3. Srisai, Dollada, Yin, Terry C, Lee, Abigail A, Grobe, Justin L, Sebag, Julien A. 2017. MRAP2 regulates ghrelin receptor signaling and hunger sensing. In Nature communications, 8, 713. doi:10.1038/s41467-017-00747-6. https://pubmed.ncbi.nlm.nih.gov/28959025/
4. Lattanzi, Roberta, Casella, Ida, Fullone, Maria Rosaria, Vincenzi, Martina, Miele, Rossella. 2024. MRAP2 Inhibits β-Arrestin-2 Recruitment to the Prokineticin Receptor 2. In Current issues in molecular biology, 46, 1607-1620. doi:10.3390/cimb46020104. https://pubmed.ncbi.nlm.nih.gov/38392222/
5. Berruien, Nasrin N A, Smith, Caroline L. 2020. Emerging roles of melanocortin receptor accessory proteins (MRAP and MRAP2) in physiology and pathophysiology. In Gene, 757, 144949. doi:10.1016/j.gene.2020.144949. https://pubmed.ncbi.nlm.nih.gov/32679290/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen