C57BL/6JCya-Cyp2g1em1/Cya
Common Name:
Cyp2g1-KO
Product ID:
S-KO-17442
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp2g1-KO
Strain ID
KOCMP-13108-Cyp2g1-B6J-VB
Gene Name
Product ID
S-KO-17442
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2g1em1/Cya mice (Catalog S-KO-17442) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040944
NCBI RefSeq
NM_013809
Target Region
Exon 4~5
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Cyp2g1 is a cytochrome P450 monooxygenase uniquely expressed in the olfactory mucosa (OM) [1,2,3,4,5,6,7,8]. It is involved in the metabolism of xenobiotic compounds and endogenous substances like testosterone and progesterone [1,3,5]. Genetic models, such as knockout mice, are valuable for studying its functions.
In Cyp2g1 -null mice, OM microsomes showed significantly lower activities in testosterone and progesterone metabolism and coumarin metabolic activation [1]. Unexpectedly, there was a significant reduction in Cyp2a5 expression in the liver, lateral nasal gland (LNG), and to a lesser extent, the kidney [1]. Although the loss of Cyp2G1 did not affect systemic clearance or hepatotoxicity of acetaminophen (AP), the LNG was protected from AP toxicity [1].
In conclusion, Cyp2g1 is crucial for the metabolism of certain compounds in the olfactory mucosa. The study of Cyp2g1 -null mice has revealed its potential role in influencing the LNG's resistance to chemical toxicity, possibly through paracrine pathways or regulation of gene expression by steroid hormones [1].
References:
1. Zhuo, Xiaoliang, Gu, Jun, Behr, Melissa J, Collins, Doris N, Ding, Xinxin. 2003. Targeted disruption of the olfactory mucosa-specific Cyp2g1 gene: impact on acetaminophen toxicity in the lateral nasal gland, and tissue-selective effects on Cyp2a5 expression. In The Journal of pharmacology and experimental therapeutics, 308, 719-28. doi:. https://pubmed.ncbi.nlm.nih.gov/14610229/
2. Zhuo, X, Schwob, J E, Swiatek, P J, Ding, X. . Mouse cyp2g1 gene: promoter structure and tissue-specific expression of a cyp2g1-lacz fusion gene in transgenic mice. In Archives of biochemistry and biophysics, 391, 127-36. doi:. https://pubmed.ncbi.nlm.nih.gov/11414693/
3. Hua, Z, Zhang, Q Y, Su, T, Lipinskas, T W, Ding, X. . cDNA cloning, heterologous expression, and characterization of mouse CYP2G1, an olfactory-specific steroid hydroxylase. In Archives of biochemistry and biophysics, 340, 208-14. doi:. https://pubmed.ncbi.nlm.nih.gov/9143323/
4. Wang, Qiang, Titlow, William B, McClintock, Declan A, Stromberg, Arnold J, McClintock, Timothy S. . Activity-Dependent Gene Expression in the Mammalian Olfactory Epithelium. In Chemical senses, 42, 611-624. doi:10.1093/chemse/bjx028. https://pubmed.ncbi.nlm.nih.gov/28525560/
5. Gu, J, Zhang, Q Y, Genter, M B, Nebert, D W, Ding, X. . Purification and characterization of heterologously expressed mouse CYP2A5 and CYP2G1: role in metabolic activation of acetaminophen and 2,6-dichlorobenzonitrile in mouse olfactory mucosal microsomes. In The Journal of pharmacology and experimental therapeutics, 285, 1287-95. doi:. https://pubmed.ncbi.nlm.nih.gov/9618435/
6. Marini, S, Longo, V, Mazzaccaro, A, Gervasi, P G. . Xenobiotic-metabolizing enzymes in pig nasal and hepatic tissues. In Xenobiotica; the fate of foreign compounds in biological systems, 28, 923-35. doi:. https://pubmed.ncbi.nlm.nih.gov/9849640/
7. Ling, Guoyu, Gu, Jun, Genter, Mary Beth, Zhuo, Xiaoliang, Ding, Xinxin. . Regulation of cytochrome P450 gene expression in the olfactory mucosa. In Chemico-biological interactions, 147, 247-58. doi:. https://pubmed.ncbi.nlm.nih.gov/15135081/
8. Yu, Tun-Tzu, McIntyre, Jeremy C, Bose, Soma C, Owen, Michael C, McClintock, Timothy S. . Differentially expressed transcripts from phenotypically identified olfactory sensory neurons. In The Journal of comparative neurology, 483, 251-62. doi:. https://pubmed.ncbi.nlm.nih.gov/15682396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen