C57BL/6JCya-Slc12a5em1/Cya
Common Name:
Slc12a5-KO
Product ID:
S-KO-17445
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slc12a5-KO
Strain ID
KOCMP-57138-Slc12a5-B6J-VA
Gene Name
Product ID
S-KO-17445
Gene Alias
KCC2; mKIAA1176
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc12a5em1/Cya mice (Catalog S-KO-17445) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099092
NCBI RefSeq
NM_020333
Target Region
Exon 2~4
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Slc12a5, encoding K+-Cl- cotransporter 2 (KCC2), is the main Cl- extruder of neurons. It enables the proper inhibitory function of neurotransmitters like γ-aminobutyric acid (GABA) and glycine, thus maintaining the excitation-inhibition balance in the nervous system. Post-translational modifications such as phosphorylation and glycosylation regulate KCC2 functions [1].
Loss-of-function variants of human SLC12A5 have pathogenic potential, as they can disrupt the Cl- transporting activity of KCC2, which may lead to neurological and psychiatric disorders [1]. In glioblastoma multiforme (GBM), SLC12A5 shows promise as a novel biomarker, being deficient in GBM but enriched in normal neural tissues, and its overexpression inhibits GBM cell proliferation [2]. In hepatocellular carcinoma (HCC), high SLC12A5 expression promotes tumor growth and ferroptosis resistance by inducing ER stress and changing cystine transport [3]. In bladder urothelial carcinoma, SLC12A5 promotes tumor progression by interacting with and stabilizing SOX18, then upregulating MMP7 [4]. In breast cancer, ETV4-mediated transcriptional activation of SLC12A5 exacerbates ferroptosis resistance and glucose metabolism reprogramming [5]. In prostate cancer, SLC12A5 functions as an oncogene to promote tumor progression and castration resistance through YTHDC1 and HOXB13 [6]. In ovarian carcinoma, overexpression of SLC12A5 is associated with tumor progression and poor survival [7].
In conclusion, Slc12a5 plays a crucial role in maintaining the normal inhibitory function of neurons. Its dysregulation is associated with various cancers, including GBM, HCC, bladder, breast, prostate, and ovarian carcinomas. Research on Slc12a5, especially through gene-knockout or conditional-knockout mouse models (not directly mentioned in the references but relevant for further functional studies), could provide more insights into its functions and its roles in disease, potentially leading to new therapeutic strategies for these disorders.
References:
1. Fukuda, Atsuo, Watanabe, Miho. 2018. Pathogenic potential of human SLC12A5 variants causing KCC2 dysfunction. In Brain research, 1710, 1-7. doi:10.1016/j.brainres.2018.12.025. https://pubmed.ncbi.nlm.nih.gov/30576625/
2. Chen, Jiakai, Wang, Handong, Deng, Chulei, Fei, Maoxing. 2023. SLC12A5 as a novel potential biomarker of glioblastoma multiforme. In Molecular biology reports, 50, 4285-4299. doi:10.1007/s11033-023-08371-y. https://pubmed.ncbi.nlm.nih.gov/36917367/
3. Tong, Qing, Qin, Wei, Li, Zheng-Hao, Chu, Yuan, Xu, Xun-Di. 2023. SLC12A5 promotes hepatocellular carcinoma growth and ferroptosis resistance by inducing ER stress and cystine transport changes. In Cancer medicine, 12, 8526-8541. doi:10.1002/cam4.5605. https://pubmed.ncbi.nlm.nih.gov/36645171/
4. Wang, Long, Zhang, Qun, Wu, Pei, Liu, Bin, Liu, Jianye. 2020. SLC12A5 interacts and enhances SOX18 activity to promote bladder urothelial carcinoma progression via upregulating MMP7. In Cancer science, 111, 2349-2360. doi:10.1111/cas.14502. https://pubmed.ncbi.nlm.nih.gov/32449280/
5. Wang, Huan, Dai, Yanyan, Wang, Fengxiang. 2024. ETV4‑mediated transcriptional activation of SLC12A5 exacerbates ferroptosis resistance and glucose metabolism reprogramming in breast cancer cells. In Molecular medicine reports, 30, . doi:10.3892/mmr.2024.13341. https://pubmed.ncbi.nlm.nih.gov/39370816/
6. Yuan, Shuai, He, Shao-Hua, Li, Lu-Yao, Hu, Hailiang, Zeng, Xian-Tao. 2023. A potassium-chloride co-transporter promotes tumor progression and castration resistance of prostate cancer through m6A reader YTHDC1. In Cell death & disease, 14, 7. doi:10.1038/s41419-022-05544-8. https://pubmed.ncbi.nlm.nih.gov/36609444/
7. Yang, Gui-Ping, He, Wei-Peng, Tan, Jin-Feng, Xie, Dan, Yang, Guo-Fen. . Overexpression of SLC12A5 is associated with tumor progression and poor survival in ovarian carcinoma. In International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 29, 1280-1284. doi:10.1136/ijgc-2019-000229. https://pubmed.ncbi.nlm.nih.gov/31570543/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen