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C57BL/6JCya-Sh3bp1em1/Cya
Common Name:
Sh3bp1-KO
Product ID:
S-KO-17449
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Sh3bp1-KO
Strain ID
KOCMP-20401-Sh3bp1-B6J-VA
Gene Name
Sh3bp1
Product ID
S-KO-17449
Gene Alias
3BP-1
Background
C57BL/6JCya
NCBI ID
20401
Modification
Conventional knockout
Chromosome
15
Phenotype
MGI:104603
Document
Click here to download >>
Application
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More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sh3bp1em1/Cya mice (Catalog S-KO-17449) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001226
NCBI RefSeq
NM_001316684
Target Region
Exon 2~3
Size of Effective Region
~1.2 kb
Detailed Document
Click here to download >>
Overview of Gene Research
SH3BP1, also known as ARHGAP43, is a member of the RhoGAP family. It specifically inactivates Rac1 and its target protein Wave2, and is involved in regulating cell motility. It is associated with pathways such as the Ral/exocyst and Rac signaling pathways, playing a crucial role in many biological processes including cell-cell junction formation, and is of great importance in both normal cell functions and disease-related processes [1,3,5,6,7]. Genetic models, like KO/CKO mouse models, can potentially be used to further explore its functions.

In melanoma, overexpression of SH3BP1 promoted cell proliferation, migration, and invasion in vitro by increasing Rac1 activity and Wave2 protein levels, and facilitated melanoma progression in vivo by upregulating Wave2 protein expression, suggesting it promotes melanoma progression through the Rac1/Wave2 signaling pathway [1]. In acute myeloid leukemia, elevated SH3BP1 expression was associated with poor prognosis, and higher expression was an independent prognostic predictor. Down-regulation of its expression inhibited AML cell proliferation [2]. In cervical cancer, SH3BP1 overexpression promoted cell invasion, migration, and chemoresistance to cisplatin through increasing Rac1 activity and Wave2 protein level [3]. In chronic myeloid leukemia, Cobll1 preferentially binds to PACSIN2, releasing SH3BP1 to promote the SH3BP1/Rac1 pathway, suppress TKI-mediated apoptosis, and lead to TKI resistance [4]. In hepatocellular carcinoma, SH3BP1 overexpressed in HCC tissues and highly metastatic HCC cells, promoted VEGF secretion via Rac1-WAVE2 signaling, and contributed to tumor invasion, microvessel formation, metastasis, and recurrence [6].

In conclusion, SH3BP1 is essential in regulating cell motility through its impact on the Rac1/Wave2 pathway. Model-based research, such as studies on its over-or under-expression in various cell lines and in vivo models, has revealed its significant role in multiple cancer-related processes, including melanoma, acute myeloid leukemia, cervical cancer, chronic myeloid leukemia, and hepatocellular carcinoma. These findings provide potential therapeutic targets for these diseases.

References:
1. Sun, Ting, Tong, Wenxian, Pu, Jie, Yu, Zhiguo, Kang, Zhengchun. 2023. SH3BP1 Regulates Melanoma Progression Through Race1/Wace2 Signaling Pathway. In Clinical Medicine Insights. Oncology, 17, 11795549231168075. doi:10.1177/11795549231168075. https://pubmed.ncbi.nlm.nih.gov/37114076/
2. Yang, Li, Xu, Qiang, Li, Junnan. 2024. Prognostic impact of ARHGAP43(SH3BP1) in acute myeloid leukemia. In Journal of the Formosan Medical Association = Taiwan yi zhi, 123, 992-1003. doi:10.1016/j.jfma.2024.04.002. https://pubmed.ncbi.nlm.nih.gov/38582737/
3. Wang, Jingjing, Feng, Yeqian, Chen, Xishan, Ma, Shuyun, Zou, Wen. 2017. SH3BP1-induced Rac-Wave2 pathway activation regulates cervical cancer cell migration, invasion, and chemoresistance to cisplatin. In Journal of cellular biochemistry, 119, 1733-1745. doi:10.1002/jcb.26334. https://pubmed.ncbi.nlm.nih.gov/28786507/
4. Park, Kibeom, Yoo, Hee-Seop, Oh, Chang-Kyu, Lee, Yoonsung, Kim, Dong-Wook. 2022. Reciprocal interactions among Cobll1, PACSIN2, and SH3BP1 regulate drug resistance in chronic myeloid leukemia. In Cancer medicine, 11, 4005-4020. doi:10.1002/cam4.4727. https://pubmed.ncbi.nlm.nih.gov/35352878/
5. Parrini, Maria Carla, Sadou-Dubourgnoux, Amel, Aoki, Kazuhiro, Rossé, Carine, Camonis, Jacques. . SH3BP1, an exocyst-associated RhoGAP, inactivates Rac1 at the front to drive cell motility. In Molecular cell, 42, 650-61. doi:10.1016/j.molcel.2011.03.032. https://pubmed.ncbi.nlm.nih.gov/21658605/
6. Tao, Yiming, Hu, Kuan, Tan, Fengbo, Luo, Jia, Wang, Zhiming. . SH3-domain binding protein 1 in the tumor microenvironment promotes hepatocellular carcinoma metastasis through WAVE2 pathway. In Oncotarget, 7, 18356-70. doi:10.18632/oncotarget.7786. https://pubmed.ncbi.nlm.nih.gov/26933917/
7. Elbediwy, Ahmed, Zihni, Ceniz, Terry, Stephen J, Matter, Karl, Balda, Maria S. 2012. Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex. In The Journal of cell biology, 198, 677-93. doi:10.1083/jcb.201202094. https://pubmed.ncbi.nlm.nih.gov/22891260/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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