Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Ctu1em1/Cya
Common Name:
Ctu1-KO
Product ID:
S-KO-17469
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ctu1-KO
Strain ID
KOCMP-233189-Ctu1-B6J-VB
Gene Name
Ctu1
Product ID
S-KO-17469
Gene Alias
Atpbd3
Background
C57BL/6JCya
NCBI ID
233189
Modification
Conventional knockout
Chromosome
7
Phenotype
MGI:2385277
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ctu1em1/Cya mice (Catalog S-KO-17469) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000038332
NCBI RefSeq
NM_145582
Target Region
Exon 3
Size of Effective Region
~2.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ctu1, a subunit of cytosolic thiouridylase, is crucial for the 2-thiolation at the 34th wobble uridine of the anticodon loop of specific tRNAs, such as tRNAGlnUUG, tRNAGluUUC, and tRNALysUUU [2,3,5]. This modification is involved in proper mRNA decoding by the ribosome, influencing translation fidelity and playing a role in pathways related to cell cycle regulation, development, and disease [2,3].

In zebrafish, deficiency of ctu1 leads to impaired angiogenesis and embryonic development, with single-cell RNA sequencing revealing cell cycle arrest, nerve development and erythrocyte differentiation defects, and attenuation of pro-angiogenic signaling pathways [2]. In nematode and fission yeast, inactivation of the Ctu1-Ctu2 complex results in loss of tRNA thiolation, thermosensitive viability decrease, ploidy abnormalities, and aberrant development, suggesting a role in maintaining genome integrity [3]. In human melanoma cells, inhibition of CTU1, along with ELP3 or CTU2, can synergize with MAPK signalling inhibition to kill cells, indicating its importance in cancer cell survival and resistance to targeted therapy [1]. In breast cancer, up-regulation of CTU1/2 sustains metastasis by supporting the translation of oncoproteins [4]. In neuroblastoma, CTU1 is identified as a prognostic-related gene, and a prognostic risk score model including CTU1 shows predictive value for patient survival [6]. In cattle, a missense variant in CTU1 may impact direct calving difficulty [7].

In summary, Ctu1 is essential for angiogenesis, embryonic development, genome integrity, and has implications in cancer and other diseases. Studies using genetic models like zebrafish, nematode, fission yeast, and in human cell lines have revealed its role in these biological processes and disease conditions, providing insights into potential therapeutic targets and prognostic markers.

References:
1. Rapino, Francesca, Delaunay, Sylvain, Rambow, Florian, Chariot, Alain, Close, Pierre. 2018. Codon-specific translation reprogramming promotes resistance to targeted therapy. In Nature, 558, 605-609. doi:10.1038/s41586-018-0243-7. https://pubmed.ncbi.nlm.nih.gov/29925953/
2. Yu, Yangziwei, Wang, Chuqiao, Wang, Yan, Du, Yibin, Zhou, Zhaoli. 2024. The conserved wobble uridine tRNA thiolase Ctu1 is required for angiogenesis and embryonic development. In PloS one, 19, e0315854. doi:10.1371/journal.pone.0315854. https://pubmed.ncbi.nlm.nih.gov/39705244/
3. Dewez, Monique, Bauer, Fanélie, Dieu, Marc, Vandenhaute, Jean, Hermand, Damien. 2008. The conserved Wobble uridine tRNA thiolase Ctu1-Ctu2 is required to maintain genome integrity. In Proceedings of the National Academy of Sciences of the United States of America, 105, 5459-64. doi:10.1073/pnas.0709404105. https://pubmed.ncbi.nlm.nih.gov/18391219/
4. Delaunay, Sylvain, Rapino, Francesca, Tharun, Lars, Chariot, Alain, Close, Pierre. 2016. Elp3 links tRNA modification to IRES-dependent translation of LEF1 to sustain metastasis in breast cancer. In The Journal of experimental medicine, 213, 2503-2523. doi:. https://pubmed.ncbi.nlm.nih.gov/27811057/
5. Liu, Yuchen, Long, Feng, Wang, Liangliang, Söll, Dieter, Whitman, William B. 2014. The putative tRNA 2-thiouridine synthetase Ncs6 is an essential sulfur carrier in Methanococcus maripaludis. In FEBS letters, 588, 873-7. doi:10.1016/j.febslet.2014.01.065. https://pubmed.ncbi.nlm.nih.gov/24530533/
6. Yang, Jun, Zhou, Jiaying, Li, Cuili, Wang, Shaohua. 2021. Integrated analysis of the functions and prognostic values of RNA-binding proteins in neuroblastoma. In PloS one, 16, e0260876. doi:10.1371/journal.pone.0260876. https://pubmed.ncbi.nlm.nih.gov/34879089/
7. Purfield, Deirdre C, Bradley, Daniel G, Evans, Ross D, Kearney, Francis J, Berry, Donagh P. 2015. Genome-wide association study for calving performance using high-density genotypes in dairy and beef cattle. In Genetics, selection, evolution : GSE, 47, 47. doi:10.1186/s12711-015-0126-4. https://pubmed.ncbi.nlm.nih.gov/26065883/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest