C57BL/6NCya-Dpep3em1/Cya
Common Name:
Dpep3-KO
Product ID:
S-KO-17473
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Dpep3-KO
Strain ID
KOCMP-71854-Dpep3-B6N-VA
Gene Name
Product ID
S-KO-17473
Gene Alias
1700018F16Rik; MBD-3; MLZ-136
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Dpep3em1/Cya mice (Catalog S-KO-17473) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034371
NCBI RefSeq
NM_027960
Target Region
Exon 2~7
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Dpep3, or dipeptidase 3, is one of three glycosylphosphatidylinositol-anchored metallopeptidases potentially involved in the hydrolytic metabolism of dipeptides [2]. Its mRNA is predominantly detected in testicular tissue in mice, and it is evolutionarily conserved among human, mouse, and other eutherians [1].
In a study using Nuclease technology-generated Dpep3 knockout (Dpep3-/-) mice, male Dpep3-/-mice were found to be fertile despite a significant reduction in sperm count. Histology of the testis and progression of meiotic prophase I showed no obvious difference between wild-type and Dpep3-/-mice, indicating that Dpep3 is not essential for male fertility in mice [1]. Structurally, DPEP3 shares a conserved, dimeric TIM (β/α)8-barrel fold with DPEP1 and DPEP2 but diverges in key active-site positions, resulting in no in vitro dipeptidase activity against various substrates, suggesting it may require an activating co-factor in vivo or be an inactive, degenerate enzyme [2].
In conclusion, although Dpep3 is mainly expressed in the testis, its absence does not render male mice infertile. The study of Dpep3 knockout mice has provided insights into its non-essential role in male fertility. The unique structural features of DPEP3 also contribute to our understanding of the differences between active and inactive membrane dipeptidases [1,2].
References:
1. Xie, Yubin, Khan, Ranjha, Wahab, Fazal, Jiang, Xiaohua, Shi, Qinghua. 2019. The testis-specifically expressed Dpep3 is not essential for male fertility in mice. In Gene, 711, 143925. doi:10.1016/j.gene.2019.06.015. https://pubmed.ncbi.nlm.nih.gov/31212048/
2. Hayashi, Kristyn, Longenecker, Kenton L, Koenig, Patrick, Stoll, Vincent, Vivona, Sandro. 2020. Structure of human DPEP3 in complex with the SC-003 antibody Fab fragment reveals basis for lack of dipeptidase activity. In Journal of structural biology, 211, 107512. doi:10.1016/j.jsb.2020.107512. https://pubmed.ncbi.nlm.nih.gov/32325220/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen