C57BL/6JCya-Kcnj11em1/Cya
Common Name:
Kcnj11-KO
Product ID:
S-KO-17480
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kcnj11-KO
Strain ID
KOCMP-16514-Kcnj11-B6J-VA
Gene Name
Product ID
S-KO-17480
Gene Alias
Kir6.2; mBIR
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kcnj11em1/Cya mice (Catalog S-KO-17480) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000211674
NCBI RefSeq
NM_010602
Target Region
Exon 1
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Kcnj11, the potassium inwardly rectifying channel, subfamily J, member 11 gene, encodes the Kir6.2 subunit of the pancreatic β -cell ATP - sensitive potassium (KATP) channel. This channel is a key component of the glucose - stimulated insulin secretion pathway, thus playing a crucial role in regulating insulin secretion and blood glucose levels [1,2].
Mutations in Kcnj11 can lead to dysregulated insulin secretion. Inactivating mutations cause an oversecretion of insulin, resulting in congenital hyperinsulinism, while activating mutations lead to diabetes [1]. A genetically engineered mouse model showed that the K23 variant of Kcnj11 (a common polymorphism) impairs glucose - induced insulin secretion, increases diabetes risk when combined with a high - fat diet and obesity, and accelerates diet - induced diabetes progression [3].
In conclusion, Kcnj11 is essential for normal insulin secretion through its role in the KATP channel of pancreatic β - cells. Studies using mouse models, like the one with the K23 variant, have revealed its significant contribution to diabetes - related disease processes, enhancing our understanding of the genetic mechanisms underlying diabetes and potentially guiding the development of targeted therapies.
References:
1. De Franco, Elisa, Saint-Martin, Cécile, Brusgaard, Klaus, Bellanné-Chantelot, Christine, Flanagan, Sarah E. 2020. Update of variants identified in the pancreatic β-cell KATP channel genes KCNJ11 and ABCC8 in individuals with congenital hyperinsulinism and diabetes. In Human mutation, 41, 884-905. doi:10.1002/humu.23995. https://pubmed.ncbi.nlm.nih.gov/32027066/
2. Haghvirdizadeh, Polin, Mohamed, Zahurin, Abdullah, Nor Azizan, Haerian, Monir Sadat, Haerian, Batoul Sadat. 2015. KCNJ11: Genetic Polymorphisms and Risk of Diabetes Mellitus. In Journal of diabetes research, 2015, 908152. doi:10.1155/2015/908152. https://pubmed.ncbi.nlm.nih.gov/26448950/
3. Sachse, Gregor, Haythorne, Elizabeth, Hill, Thomas, Cox, Roger D, Ashcroft, Frances M. 2021. The KCNJ11-E23K Gene Variant Hastens Diabetes Progression by Impairing Glucose-Induced Insulin Secretion. In Diabetes, 70, 1145-1156. doi:10.2337/db20-0691. https://pubmed.ncbi.nlm.nih.gov/33568422/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen