C57BL/6JCya-Minar2em1/Cya
Common Name:
Minar2-KO
Product ID:
S-KO-17487
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Minar2-KO
Strain ID
KOCMP-225583-Minar2-B6J-VB
Gene Name
Product ID
S-KO-17487
Gene Alias
A730017C20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Minar2em1/Cya mice (Catalog S-KO-17487) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165666
NCBI RefSeq
NM_173759.5
Target Region
Exon 1~3
Size of Effective Region
~14.7 kb
Detailed Document
Overview of Gene Research
Minar2, also known as Kiaa1024L, is a gene that plays essential roles in multiple biological processes. It is involved in regulating cholesterol distribution and homeostasis, and is associated with the mammalian TOR complex 1 (mTORC1) pathway. Its proper function is crucial for normal hearing, metabolism, and motor function [1,2,4]. Genetic models, especially mouse models, have been invaluable in studying Minar2's functions.
In gene knockout (KO) mouse models, inactivation of Minar2 leads to various phenotypes. Minar2 KO mice exhibit increased body fat with hypertrophic adipocytes, develop obesity and impaired glucose tolerance on a high - fat diet, as Minar2 interacts with Raptor to inhibit mTOR activation and its inactivation hyperactivates mTOR signaling [2]. In addition, Minar2 KO mice show severe motor deficits similar to Parkinson's disease - like symptoms, with loss of tyrosine hydroxylase - positive neurons and up - regulation of α - synuclein protein expression [4]. In zebrafish, disruption of kiaa1024L/minar2 causes hearing loss, along with defects in mechanotransduction, abnormal hair bundles, and enlarged apical lysosomes in hair cells, as it regulates cholesterol localization to hair bundles [1]. Mutations in MINAR2 also cause deafness in humans and mice, with Minar2 expressed in the inner ear and its loss leading to sensorineural hearing loss [3].
In conclusion, Minar2 is essential for normal physiological functions. The study of Minar2 KO mouse models has revealed its significance in obesity, metabolic disorders, Parkinson's - like symptoms, and hearing. Understanding Minar2's functions provides insights into the underlying mechanisms of these diseases and may potentially lead to novel treatment strategies.
References:
1. Gao, Ge, Guo, Shuyu, Zhang, Quan, Zhang, Cuizhen, Peng, Gang. 2022. Kiaa1024L/Minar2 is essential for hearing by regulating cholesterol distribution in hair bundles. In eLife, 11, . doi:10.7554/eLife.80865. https://pubmed.ncbi.nlm.nih.gov/36317962/
2. Lotfollahzadeh, Saran, Xia, Chaoshuang, Amraei, Razie, Chitalia, Vipul, Rahimi, Nader. 2023. Inactivation of Minar2 in mice hyperactivates mTOR signaling and results in obesity. In Molecular metabolism, 73, 101744. doi:10.1016/j.molmet.2023.101744. https://pubmed.ncbi.nlm.nih.gov/37245847/
3. Bademci, Guney, Lachgar-Ruiz, María, Deokar, Mangesh, Nasir, Jamal, Tekin, Mustafa. 2022. Mutations in MINAR2 encoding membrane integral NOTCH2-associated receptor 2 cause deafness in humans and mice. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2204084119. doi:10.1073/pnas.2204084119. https://pubmed.ncbi.nlm.nih.gov/35727972/
4. Ho, Rachel Xi-Yeen, Amraei, Razie, De La Cena, Kyle Oliver Corcino, Chitalia, Vipul, Rahimi, Nader. 2020. Loss of MINAR2 impairs motor function and causes Parkinson's disease-like symptoms in mice. In Brain communications, 2, fcaa047. doi:10.1093/braincomms/fcaa047. https://pubmed.ncbi.nlm.nih.gov/32954300/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen