C57BL/6JCya-Hrobem1/Cya
Common Name:
Hrob-KO
Product ID:
S-KO-17488
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hrob-KO
Strain ID
KOCMP-217216-Hrob-B6J-VB
Gene Name
Product ID
S-KO-17488
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hrobem1/Cya mice (Catalog S-KO-17488) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100392
NCBI RefSeq
NM_153544
Target Region
Exon 6~10
Size of Effective Region
~6.3 kb
Detailed Document
Overview of Gene Research
HROB, also known as C17orf53, is an OB - fold - containing factor with a crucial role in DNA repair and replication. It is involved in the homologous recombination (HR) pathway and DNA inter - strand crosslink (ICL) repair. HROB recruits the MCM8 - MCM9 helicase to DNA damage sites, promoting DNA synthesis during HR and DNA unwinding downstream of MCM8 - 9 loading and ring formation on single - stranded DNA [2,1,3,4]. This function is essential for maintaining genomic integrity, tumor suppression, and gamete formation [2].
Mice with targeted mutations in Hrob are infertile due to germ cell depletion and show phenotypes consistent with prophase I meiotic arrest, indicating HROB's importance in meiosis [2]. In humans, variants in HROB are associated with premature ovarian insufficiency, suggesting its role in ovarian function [5,6].
In summary, HROB is vital for DNA repair and replication processes, especially in homologous recombination. Its function in maintaining genomic integrity and role in meiosis and ovarian function are revealed through mouse models and human genetic studies. Understanding HROB may provide insights into diseases related to DNA damage and infertility.
References：
1. Acharya, Ananya, Bret, Hélène, Huang, Jen-Wei, Guérois, Raphaël, Cejka, Petr. 2024. Mechanism of DNA unwinding by MCM8-9 in complex with HROB. In Nature communications, 15, 3584. doi:10.1038/s41467-024-47936-8. https://pubmed.ncbi.nlm.nih.gov/38678026/
2. Hustedt, Nicole, Saito, Yuichiro, Zimmermann, Michal, Jurisicova, Andrea, Durocher, Daniel. 2019. Control of homologous recombination by the HROB-MCM8-MCM9 pathway. In Genes & development, 33, 1397-1415. doi:10.1101/gad.329508.119. https://pubmed.ncbi.nlm.nih.gov/31467087/
3. Acharya, Ananya, Bret, Hélène, Huang, Jen-Wei, Guérois, Raphaël, Cejka, Petr. 2023. Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.06.12.544631. https://pubmed.ncbi.nlm.nih.gov/37398313/
4. Acharya, Ananya, Bret, Hélène, Huang, Jen-Wei, Guérois, Raphaël, Cejka, Petr. 2023. Mechanism of DNA unwinding by hexameric MCM8-9 in complex with HROB. In Research square, , . doi:10.21203/rs.3.rs-3054483/v1. https://pubmed.ncbi.nlm.nih.gov/37461676/
5. Tucker, Elena J, Bell, Katrina M, Robevska, Gorjana, Jaillard, Sylvie, Sinclair, Andrew H. 2021. Meiotic genes in premature ovarian insufficiency: variants in HROB and REC8 as likely genetic causes. In European journal of human genetics : EJHG, 30, 219-228. doi:10.1038/s41431-021-00977-9. https://pubmed.ncbi.nlm.nih.gov/34707299/
6. Wu, Xinghan, Peng, Xiangyun, Zheng, Yu, Peng, Yu, Wang, Hua. 2023. Genetic analysis of novel pathogenic gene HROB in a family with primary ovarian insufficiency. In Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 52, 727-731. doi:10.3724/zdxbyxb-2023-0468. https://pubmed.ncbi.nlm.nih.gov/38105698/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen