C57BL/6JCya-Ass1em1/Cya
Common Name:
Ass1-KO
Product ID:
S-KO-17507
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ass1-KO
Strain ID
KOCMP-11898-Ass1-B6J-VA
Gene Name
Product ID
S-KO-17507
Gene Alias
ASS; Ass-1; fold
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ass1em1/Cya mice (Catalog S-KO-17507) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102840
NCBI RefSeq
NM_007494
Target Region
Exon 8
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
ASS1, also known as argininosuccinate synthetase 1, is a key enzyme in the urea cycle and the citrulline - nitric oxide cycle. It converts citrulline into argininosuccinate. This enzyme's function is crucial for maintaining nitrogen balance and is involved in various biological processes related to metabolism and immune response [1,5].
In macrophages, IFN - γ and/or LPS stimulation promotes STAT1 - mediated ASS1 transcription and JAK2 - mediated phosphorylation of ASS1 at tyrosine 87, leading to citrulline depletion, which is required for efficient proinflammatory signaling activation. Blockage of ASS1 - mediated citrulline depletion suppresses the host defense against bacterial infection in vivo, highlighting its role in innate immune - signaling [1].
In non - small cell lung cancer cells, loss of ASS1 increases sensitivity to erastin - induced ferroptosis both in vitro and in vivo, as ASS1 promotes reductive carboxylation of cytosolic glutamine and confers ferroptosis resistance [2].
In ovarian cancer, disruption of the ASS1 - related AMP/ATP balance activates AMPK and CPT1A, enhancing fatty acid oxidation (FAO) and anoikis resistance [3].
In colon cancer cells, prolonged loss of ASS1 promotes DNA damage, and following DNA damage, ASS1 is part of the p53 network that pauses cell cycle progression [4].
In triple - negative breast cancer, ASS1 binds to PHGDH and promotes its degradation to inhibit serine synthesis and tumorigenesis [6].
In the liver, down - regulation of ASS1 in the TAA - induced liver injury model is rescued by activation of FXR, which promotes ASS1 transcription and alleviates liver injury [7].
In conclusion, ASS1 is essential in multiple biological processes. Its role in immune response, cancer cell survival, and liver injury has been revealed through in vivo studies including gene - knockout (KO) or conditional - knockout (CKO) mouse models in relevant disease areas such as bacterial infection, cancer, and acute liver injury. These findings contribute to understanding the mechanisms of disease occurrence and may provide new therapeutic targets.
References：
1. Mao, Youxiang, Shi, Di, Li, Gen, Jiang, Peng. 2022. Citrulline depletion by ASS1 is required for proinflammatory macrophage activation and immune responses. In Molecular cell, 82, 527-541.e7. doi:10.1016/j.molcel.2021.12.006. https://pubmed.ncbi.nlm.nih.gov/35016033/
2. Hu, Qiangsheng, Dai, Jie, Zhang, Zheng, Zhang, Lele, Zhang, Peng. . ASS1-Mediated Reductive Carboxylation of Cytosolic Glutamine Confers Ferroptosis Resistance in Cancer Cells. In Cancer research, 83, 1646-1665. doi:10.1158/0008-5472.CAN-22-1999. https://pubmed.ncbi.nlm.nih.gov/36892426/
3. Feng, Xu, Ji, Zhaodong, Fan, Xiaoxi, Ren, Chunxia, Yang, Gong. 2024. ASS1 enhances anoikis resistance via AMPK/CPT1A-mediated fatty acid metabolism in ovarian cancer. In Cancer letters, , 217082. doi:10.1016/j.canlet.2024.217082. https://pubmed.ncbi.nlm.nih.gov/38914306/
4. Lim, Lisha Qiu Jin, Adler, Lital, Hajaj, Emma, Ulitsky, Igor, Erez, Ayelet. 2024. ASS1 metabolically contributes to the nuclear and cytosolic p53-mediated DNA damage response. In Nature metabolism, 6, 1294-1309. doi:10.1038/s42255-024-01060-5. https://pubmed.ncbi.nlm.nih.gov/38858597/
5. Diez-Fernandez, Carmen, Rüfenacht, Véronique, Häberle, Johannes. 2017. Mutations in the Human Argininosuccinate Synthetase (ASS1) Gene, Impact on Patients, Common Changes, and Structural Considerations. In Human mutation, 38, 471-484. doi:10.1002/humu.23184. https://pubmed.ncbi.nlm.nih.gov/28111830/
6. Luo, Wensong, Zou, Zizheng, Nie, Yuan, Zhou, Jiang, Luo, Zhiyong. 2024. ASS1 inhibits triple-negative breast cancer by regulating PHGDH stability and de novo serine synthesis. In Cell death & disease, 15, 319. doi:10.1038/s41419-024-06672-z. https://pubmed.ncbi.nlm.nih.gov/38710705/
7. Hong, Weilong, Peng, Xuyun, Zhou, Xue, Ye, Zhiqiang, Liang, Weicheng. 2022. FXR/ASS1 axis attenuates the TAA-induced liver injury through arginine metabolism. In Biochemical and biophysical research communications, 611, 31-37. doi:10.1016/j.bbrc.2022.04.073. https://pubmed.ncbi.nlm.nih.gov/35477090/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen