C57BL/6JCya-Sephs1em1/Cya
Common Name:
Sephs1-KO
Product ID:
S-KO-17523
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sephs1-KO
Strain ID
KOCMP-109079-Sephs1-B6J-VB
Gene Name
Product ID
S-KO-17523
Gene Alias
1110046B24Rik; SELD; SPS; SPS1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sephs1em1/Cya mice (Catalog S-KO-17523) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027973
NCBI RefSeq
NM_175400
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
SEPHS1, short for Selenophosphate Synthetase 1, is an enzyme-encoding gene. It is crucial in the synthesis of selenophosphate, the active donor of selenium required for selenoprotein biosynthesis [1,2,3,8]. Selenoproteins are involved in antioxidant defense, thyroid hormone metabolism, and cellular homeostasis [1]. The gene has been associated with multiple biological processes and disease-related pathways, making it a subject of significant interest in understanding normal development and disease pathogenesis [1,2,3,4,5,6,7,9]. Genetic models, such as knockout (KO) mouse models, have been instrumental in exploring its functions [4,7,8].
In mouse models, SEPHS1 deficiency leads to various consequences. In embryonic stem cells, SEPHS1 KO has little effect on pluripotency maintenance but impairs differentiation into three germ layers and gastruloid aggregation, especially cardiac differentiation [4]. In adult mice, cartilage-specific Sephs1 knockout causes aging-associated osteoarthritis and augments post-traumatic OA, as SEPHS1 downregulation in chondrocytes elevates reactive oxygen species (ROS) levels and facilitates chondrocyte senescence [3]. SEPHS1 deficiency in mouse embryos affects retinoic signaling and other related signaling pathways, leading to embryonic lethality at E11.5 [2]. In endothelial cells, Sephs1-knockout results in the accumulation of superoxide and lipid peroxide, reduction in nitric oxide, inhibition of cell proliferation and angiogenic tube formation, and cell cycle arrest at G2/M phase [7]. In Drosophila and mice, disruption of the SEPHS1 gene leads to inhibition of cell proliferation, embryonic lethality, and morphological changes [8].
In conclusion, SEPHS1 is essential for various biological processes, including redox homeostasis, cell proliferation, and differentiation. Its deficiency in KO/CKO mouse models has been linked to multiple disease-like conditions such as neurodevelopmental disorders, osteoarthritis, and endothelial cell dysfunction, highlighting its importance in understanding these disease mechanisms.
References:
1. Ahmed Mohamed, Zakaria, Yang, Jianli, Wen, Jianping, Jia, Feiyong, Banerjee, Santasree. 2024. SEPHS1 Gene: A new master key for neurodevelopmental disorders. In Clinica chimica acta; international journal of clinical chemistry, 562, 119844. doi:10.1016/j.cca.2024.119844. https://pubmed.ncbi.nlm.nih.gov/38960024/
2. Bang, Jeyoung, Kang, Donghyun, Jung, Jisu, Kim, Jin-Hong, Lee, Byeong Jae. 2022. SEPHS1: Its evolution, function and roles in development and diseases. In Archives of biochemistry and biophysics, 730, 109426. doi:10.1016/j.abb.2022.109426. https://pubmed.ncbi.nlm.nih.gov/36202216/
3. Kang, Donghyun, Lee, Jeeyeon, Jung, Jisu, Lee, Byeong Jae, Kim, Jin-Hong. 2022. Selenophosphate synthetase 1 deficiency exacerbates osteoarthritis by dysregulating redox homeostasis. In Nature communications, 13, 779. doi:10.1038/s41467-022-28385-7. https://pubmed.ncbi.nlm.nih.gov/35140209/
4. Qiao, Lu, Dho, So Hee, Kim, Ji Young, Kim, Lark Kyun. 2021. SEPHS1 is dispensable for pluripotency maintenance but indispensable for cardiac differentiation in mouse embryonic stem cells. In Biochemical and biophysical research communications, 590, 125-131. doi:10.1016/j.bbrc.2021.12.091. https://pubmed.ncbi.nlm.nih.gov/34974300/
5. Yang, Shu, Zhang, Hongying, Yang, Hua, Jiang, Yangfu, Hua, Hui. 2021. SEPHS1 promotes SMAD2/3/4 expression and hepatocellular carcinoma cells invasion. In Experimental hematology & oncology, 10, 17. doi:10.1186/s40164-021-00212-7. https://pubmed.ncbi.nlm.nih.gov/33622411/
6. Hu, Tao, Shi, Zhongming, Sun, Yongjin, Zhang, Feng, Zhang, Wen-Zhi. 2023. SEPHS1 attenuates intervertebral disc degeneration by delaying nucleus pulposus cell senescence through the Hippo-Yap/Taz pathway. In American journal of physiology. Cell physiology, 326, C386-C399. doi:10.1152/ajpcell.00571.2023. https://pubmed.ncbi.nlm.nih.gov/38105759/
7. Jung, Jisu, Kim, Yoomin, Na, Jiwoon, Kim, Jin-Hong, Lee, Byeong Jae. 2021. Constitutive Oxidative Stress by SEPHS1 Deficiency Induces Endothelial Cell Dysfunction. In International journal of molecular sciences, 22, . doi:10.3390/ijms222111646. https://pubmed.ncbi.nlm.nih.gov/34769076/
8. Na, Jiwoon, Jung, Jisu, Bang, Jeyoung, Hatfield, Dolph L, Lee, Byeong Jae. 2018. Selenophosphate synthetase 1 and its role in redox homeostasis, defense and proliferation. In Free radical biology & medicine, 127, 190-197. doi:10.1016/j.freeradbiomed.2018.04.577. https://pubmed.ncbi.nlm.nih.gov/29715549/
9. Mullegama, Sureni V, Kiernan, Kaitlyn A, Torti, Erin, Yang, Jun, Juusola, Jane. 2024. De novo missense variants in exon 9 of SEPHS1 cause a neurodevelopmental condition with developmental delay, poor growth, hypotonia, and dysmorphic features. In American journal of human genetics, 111, 778-790. doi:10.1016/j.ajhg.2024.02.016. https://pubmed.ncbi.nlm.nih.gov/38531365/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen