C57BL/6JCya-Ltbem1/Cya
Common Name:
Ltb-KO
Product ID:
S-KO-17527
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ltb-KO
Strain ID
KOCMP-16994-Ltb-B6J-VB
Gene Name
Product ID
S-KO-17527
Gene Alias
LTbeta; Tnfc; Tnfsf3; Tnlg1c; p33
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ltbem1/Cya mice (Catalog S-KO-17527) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025262
NCBI RefSeq
NM_008518
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Ltb, related to leukotriene B4 (LTB4) which is a lipid mediator produced from arachidonic acid, plays a crucial role in inflammatory processes. LTB4 acts as a chemoattractant for inflammatory leukocytes, and is involved in the leukocyte recruitment, pathogen elimination, and various inflammatory diseases through its receptors BLT1 and BLT2 [2]. It also participates in a cascade with complement, lipid, cytokine and chemokine to initiate and sustain neutrophilic inflammation in inflammatory arthritis [1].
Genetic models have been instrumental in understanding Ltb's functions. For example, in BLT1 -deficient mice, the LTB4/BLT1 axis was found to enhance leukocyte recruitment to infected sites, highlighting its role in host defense [2]. In a study of chronic granulomatous disease, antagonizing BLT1 blocked neutrophil aggregation in an ex vivo pyogranuloma formation system using peritoneal cells of CGD (gp91phox -/-) mice, suggesting LTB4's key role in pyogranuloma formation [4]. Also, in Lta4h -/- mice, blocking the LTB4 signaling enhanced the efficacy of immunotherapy against lung cancer, indicating LTB4 signaling's involvement in tumor - associated macrophages (TAMs) recruitment and immunosuppression [3].
In conclusion, Ltb, through its association with LTB4, is essential in inflammatory responses, host defense, and disease - related processes such as in inflammatory arthritis, chronic granulomatous disease, and lung cancer. Studies using gene - knockout mouse models have significantly contributed to revealing its roles in these disease areas, providing insights into potential therapeutic targets.
References:
1. Miyabe, Yoshishige, Miyabe, Chie, Luster, Andrew D. . LTB4 and BLT1 in inflammatory arthritis. In Seminars in immunology, 33, 52-57. doi:10.1016/j.smim.2017.09.009. https://pubmed.ncbi.nlm.nih.gov/29042029/
2. Saeki, Kazuko, Yokomizo, Takehiko. . Identification, signaling, and functions of LTB4 receptors. In Seminars in immunology, 33, 30-36. doi:10.1016/j.smim.2017.07.010. https://pubmed.ncbi.nlm.nih.gov/29042026/
3. Yan, Jiangna, Zhu, Jinglin, Li, Xiaolan, Huang, Chao, Zheng, Chunli. 2023. Blocking LTB4 signaling-mediated TAMs recruitment by Rhizoma Coptidis sensitizes lung cancer to immunotherapy. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 119, 154968. doi:10.1016/j.phymed.2023.154968. https://pubmed.ncbi.nlm.nih.gov/37531900/
4. Haist, Kelsey C, Gibbings, Sophie L, Jacobelli, Jordan, Henson, Peter M, Bratton, Donna L. 2024. A LTB4/CD11b self-amplifying loop drives pyogranuloma formation in chronic granulomatous disease. In iScience, 27, 109589. doi:10.1016/j.isci.2024.109589. https://pubmed.ncbi.nlm.nih.gov/38623335/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen